Human IL-5 CLIA Kit | IL-5 clia kit

IL-5 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510794

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Human
• Synonyms: IL-5; IL-5 (Human) LumiAb ELISA Kit; Human IL-5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; IL-5 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IL-33 ELISA is capable of recognizing both recombinant and naturally produced Human IL-33 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 2% cross reactivity.
• Sequence Length: 134

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-5 clia kit

Principle of the Assay: The LumiAb Human IL-33 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-33 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-33 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human IL-33 or Interleukin-33, also known as Nuclear Factor from High Endothelial Venules, is a 270 amino acid cytokine encoded by the IL33 gene at locus 9p24.1 on chromosome 9. After initial synthesis, the 111 residue propeptide is cleaved from the actual 159 residue IL-33 chain, thus allowing for the cytokine to properly fold. It is proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. IL-33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. Moreover, IL-33 is a ligand for IL-33R, an IL-1 family receptor that is selectively expressed on Th2 cells and mast cells. The cytokine binds to and signals through IL-1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4 and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14 and MAPK8. Typically, IL-33 is highly expressed in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes, but almost undetectable in placenta.

Product Categories/Family for IL-5 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.3
• UniProt Accession #: P05113
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation