Human IL-13 CLIA Kit | IL-13 clia kit

IL-13 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510780

• Gene Names: IL13; P600; IL-13
• Reactivity: Human
• Synonyms: IL-13; IL-13 (Human) LumiAb ELISA Kit; Human IL-13; Interleukin-13; NC30; IL-13 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IL-12 ELISA is capable of recognizing both recombinant and naturally produced Human IL-12 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 146

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-13 clia kit

Principle of the Assay: The LumiAb Human IL-12 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-12 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-12 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human IL-12 or Interleukin-12, also known as Natural Killer Cell Stimulatory Factor is composed of 2 subunits, encoded by the IL12A and IL12B genes located at loci 3q25.33-3q26 and 5q31.1-5q33.1 on chromosome 3 and 5, respectively. Composed of 2 subunits at 219 and 328 residues, respectively, the IL-12 heterodimeric cytokine can act as a growth factor for activated T-and NK-cells, enhance the lytic activity of NK/lymphokine-activated killer cells and stimulate the production of IFN-gamma by resting PBMC. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric Oxide Synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. The 2nd subunit of IL-12 can associate with IL-23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL-12RB1 and IL23R, activates the JAK-STAT signaling cascade, stimulates memory rather than naïve T-cells and promotes the production of pro-inflammatory cytokines. Furthermore, defects in IL-12B are a cause of Mendellian susceptibility to mycobacterial disease; also known as familial disseminated atypical mycobacterial infection while genetic variations in IL-12B are a cause of susceptibility to psoriasis type 11 (PSORS11).

Product Categories/Family for IL-13 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 26787978
• NCBI GeneID: 3596
• NCBI Accession #: NP_002179.2
• NCBI GenBank Nucleotide #: NM_002188.3
• UniProt Accession #: P35225
• NCBI Official Full Name: interleukin-13 isoform 1
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: IL13
• NCBI Official Synonym Symbols: P600; IL-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• UniProt Gene Name: IL13
• UniProt Synonym Gene Names: NC30; IL-13
• UniProt Entry Name: IL13_HUMAN

NCBI Description

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: protein binding; interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; response to lipopolysaccharide; signal transduction; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; cell-cell signaling; positive regulation of protein secretion; positive regulation of B cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; inflammatory response; cell motility; positive regulation of macrophage activation

Disease: Asthma, Susceptibility To; Allergic Rhinitis