Mouse IL-13 CLIA Kit | IL-13 clia kit

IL-13 (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510834

• Gene Names: Il13; Il-13
• Reactivity: Mouse
• Synonyms: IL-13; IL-13 (Murine) LumiAb ELISA Kit; Murine IL-13; Interleukin-13; T-cell activation protein P600; IL-13 clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine G-CSF ELISA is capable of recognizing both recombinant and naturally produced Murine G-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 131

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-13 clia kit

Principle of the Assay: The LumiAb Murine G-CSF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine G-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine G-CSF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine G-CSF, also known as Granulocyte Colony-Stimulating Factor, is a 208 amino acid cytokine protein encoded by the Csf3 gene located at chromosome 11. After initial synthesis and translocation, the 30 residue signal sequence is cleaved from the N-terminal region, allowing the 178 residue G-CSF peptide to fold properly into its mature form. Granulocyte or macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belonging to the IL-6 superfamily, monomeric G-CSF has the ability to induce granulocytes.

Product Categories/Family for IL-13 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6680403
• NCBI GeneID: 16163
• NCBI Accession #: NP_032381.1
• NCBI GenBank Nucleotide #: NM_008355.3
• UniProt Accession #: P20109
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: Il13
• NCBI Official Synonym Symbols: Il-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• UniProt Gene Name: Il13
• UniProt Synonym Gene Names: Il-13; IL-13
• UniProt Entry Name: IL13_MOUSE

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: interleukin-13 receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; positive regulation of protein secretion; positive regulation of B cell proliferation; positive regulation of ion transport; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; positive regulation of macrophage activation