Human IL-28B CLIA Kit | IL-28B clia kit

IL-28B (Human) LumiAb ELISA Kit

Cat. Num. AAA9510901

• Gene Names: IFNL3; IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• Reactivity: Human
• Synonyms: IL-28B; IL-28B (Human) LumiAb ELISA Kit; Human IL-28B; Interleukin-28B; CytLumine Zcyto22; Interferon lambda-3; Interferon lambda-4; Interleukin-28C; IL-28B clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IL-23 ELISA is capable of recognizing both recombinant and naturally produced Human IL-23 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 196

• Assay Type: Quantitative Sandwich
• Sensitivity: 31 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-28B clia kit

Principle of the Assay: The LumiAb Human IL-23 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-23 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-23 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. IL-23 associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity. IL-23 may constitute with IL-17, an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells, and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. IL-23 forms disulfide-linked heterodimer with IL12B. The heterodimer is known as interleukin IL-23 and is secreted by activated dendritic and phagocytic cells and keratinocytes. Interleukin IL-23 is expressed by dermal Langerhans cells (at protein level), up-regulated by a wide array of pathogens and pathogen-products together with self-signals for danger or injury, and up-regulated in psoriatic dermal tissues, in dendritic cells of multiple sclerosis patients and in tumors.

Product Categories/Family for IL-28B clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 28144901
• NCBI GeneID: 282617
• NCBI Accession #: NP_742151.2
• NCBI GenBank Nucleotide #: NM_172139.3
• UniProt Accession #: Q8IZI9
• NCBI Official Full Name: interferon lambda-3 isoform 2
• NCBI Official Synonym Full Names: interferon lambda 3
• NCBI Official Symbol: IFNL3
• NCBI Official Synonym Symbols: IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• NCBI Protein Information: interferon lambda-3
• UniProt Protein Name: Interferon lambda-3
• UniProt Gene Name: IFNL3
• UniProt Synonym Gene Names: IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C
• UniProt Entry Name: IFNL3_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: positive regulation of immune response; negative regulation of viral genome replication; JAK-STAT cascade; defense response to virus

Disease: Hepatitis C Virus, Susceptibility To