Human IL-17E CLIA Kit | IL-17E clia kit

IL-17E (Human) LumiAb ELISA Kit

Cat. Num. AAA9510782

• Gene Names: IL25; IL17E
• Reactivity: Human
• Synonyms: IL-17E; IL-17E (Human) LumiAb ELISA Kit; Human IL-17E; Interleukin-17E; Interleukin-25; IL-17E clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IL-17A ELISA is capable of recognizing both recombinant and naturally produced Human IL-17A proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 161

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-17E clia kit

Principle of the Assay: The LumiAb Human IL-17A Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-17A concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-17A while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human IL-17A, also known as Interleukin-17A, is a 155 amino acid cytokine encoded by the IL17A gene located at locus 6p12 on chromosome 6. After initial synthesis, the protein is proteolytically cleaved into the 23 residue signal peptide and the 132 residue IL-17A mature protein. In terms of secondary structure, the mature IL-17A cytokine contains 2 alpha helices of 3 to 4 residues each followed by 7 beta strands composed of 3 to 9 residues per strand. IL-17A is a pro-inflammatory cytokine produced by activated T-cells that can regulate the activities of nuclear factor kappa B (NF-kappaB) and mitogen-activated protein (MAP) kinases as well as induce stromal cells to produce other pro-inflammatory and hematopoietic cytokines. The cytokine can also stimulate the expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts, IL-6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). Furthermore, high levels of IL-17A are typically associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Product Categories/Family for IL-17E clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 27477081
• NCBI GeneID: 64806
• NCBI Accession #: NP_758525.1
• NCBI GenBank Nucleotide #: NM_172314.1
• UniProt Accession #: Q9H293
• NCBI Official Full Name: interleukin-25 isoform 2
• NCBI Official Synonym Full Names: interleukin 25
• NCBI Official Symbol: IL25
• NCBI Official Synonym Symbols: IL17E
• NCBI Protein Information: interleukin-25
• UniProt Protein Name: Interleukin-25
• UniProt Gene Name: IL25
• UniProt Synonym Gene Names: IL17E; IL-25; IL-17E
• UniProt Entry Name: IL25_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter