Human EGF CLIA Kit | EGF clia kit

EGF (Human) LumiAb ELISA Kit

Cat. Num. AAA9510766

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human
• Synonyms: EGF; EGF (Human) LumiAb ELISA Kit; Human EGF; Epidermal growth factor; Urogastrone; EGF clia kit

• Reactivity: Human
• Specificity: The LumiAb Human CTGF ELISA is capable of recognizing both recombinant and naturally produced Human CTGF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 1207

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EGF clia kit

Principle of the Assay: The LumiAb Human CTGF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human CTGF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human CTGF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human CTGF, also known as Connective Tissue Growth Factor, is a 349 amino acid growth factor encoded by the CTGF gene located at locus 6q23.1 on chromosome 6. Following translation, CTGF undergoes proteolytic processing where the 26 residue signal sequence is cleaved and CTGF is allowed to fold into its mature form. CTGF is characterized as a major connective tissue mitoattractant secreted by vascular endothelial cells. These growth factors typically are known to promote proliferation and differentiation of chondrocytes, mediate heparin-and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Moreover, they tend to enhance fibroblast growth factor-induced DNA synthesis. Belonging to the CCN family, the CTGF protein contains a C-terminal cysteine knot-like domain (CTCK), an IGFBP N-terminal domain, a TSP type-1 domain, and a VWFC domain. Furthermore, this particular growth factor is mainly expressed in bone marrow and thymic cells.

Product Categories/Family for EGF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 251757262
• NCBI GeneID: 1950
• UniProt Accession #: P01133
• NCBI Official Full Name: Pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]