Human TGFbeta-R3 CLIA Kit | TGFbeta-R3 clia kit

TGFbeta-R3 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510920

• Gene Names: TGFBR3; BGCAN; betaglycan
• Reactivity: Human
• Synonyms: TGFbeta-R3; TGFbeta-R3 (Human) LumiAb ELISA Kit; Human TGFbeta-R3; Transforming growth factor beta receptor type 3; Betaglycan; Transforming growth factor beta receptor III; TGFbeta-R3 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human TGF-beta3 ELISA is capable of recognizing both recombinant and naturally produced Human TGF-beta3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 850

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFbeta-R3 clia kit

Principle of the Assay: The LumiAb Human TGF-beta3 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human TGF-beta3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human TGF-beta3 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human TGF-beta3 encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1 while defects in TGF-beta3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1), also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.

Product Categories/Family for TGFbeta-R3 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 307574634
• NCBI GeneID: 7049
• NCBI Accession #: NP_001182613.1
• NCBI GenBank Nucleotide #: NM_001195684.1
• UniProt Accession #: Q03167
• NCBI Official Full Name: transforming growth factor beta receptor type 3 isoform b
• NCBI Official Synonym Full Names: transforming growth factor beta receptor 3
• NCBI Official Symbol: TGFBR3
• NCBI Official Synonym Symbols: BGCAN; betaglycan
• NCBI Protein Information: transforming growth factor beta receptor type 3
• UniProt Protein Name: Transforming growth factor beta receptor type 3
• UniProt Gene Name: TGFBR3
• UniProt Synonym Gene Names: TGF-beta receptor type 3; TGFR-3
• UniProt Entry Name: TGBR3_HUMAN

NCBI Description

This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.[provided by RefSeq, Sep 2010]

Uniprot Description

TGFBR3: Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. Interacts with TCTEX1D4. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; integral to plasma membrane; endoplasmic reticulum; receptor complex; external side of plasma membrane

Molecular Function: heparin binding; transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; fibroblast growth factor binding; transforming growth factor beta binding; punt binding; activin binding; coreceptor activity; SMAD binding; transforming growth factor beta receptor binding; PDZ domain binding

Biological Process: transforming growth factor beta receptor complex assembly; heart morphogenesis; blood vessel development; cell migration; blastocyst development; organ regeneration; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of cell motility; cardiac muscle cell proliferation; palate development; liver development; activation of NF-kappaB transcription factor; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; regulation of protein binding; response to hypoxia; ventricular cardiac muscle morphogenesis; epithelial to mesenchymal transition; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell growth; response to follicle-stimulating hormone stimulus; negative regulation of epithelial cell proliferation