Human beta-NGF CLIA Kit | beta-NGF clia kit

beta-NGF (Human) LumiAb ELISA Kit

Cat. Num. AAA9510761

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Reactivity: Human
• Synonyms: beta-NGF; beta-NGF (Human) LumiAb ELISA Kit; Human beta-NGF; Beta-nerve growth factor; beta-NGF clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Betacellulin ELISA is capable of recognizing both recombinant and naturally produced Human Betacellulin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 241

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for beta-NGF clia kit

Principle of the Assay: The LumiAb Human Betacellulin Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Betacellulin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Betacellulin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human Betacellulin is a 48 amino acid cytokine protein that is encoded by the BTC gene on chromosome 4 at locus 4q13-q21. The protein Probetacellulin is a 178 residue transmembrane precursor peptide that undergoes proteolytic cleavage, resulting in the mature form of Betacellulin. It has been determined that Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells via its high affinity interaction with the EGF receptor as it contains an EGF-like domain. The small peptide is expressed in several tissue types-predominantly in the pancreas, small intestine and range of tumor cells. The secondary structure of Betacellulin contains a 3 residue alpha helix and 5 beta strands composed of 18 residues.

Product Categories/Family for beta-NGF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 90110037
• NCBI GeneID: 4803
• UniProt Accession #: P01138
• NCBI Official Full Name: Beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity

Biological Process: response to nicotine; circadian rhythm; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of axonogenesis; positive regulation of transcription factor activity; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V