Human BD-2 CLIA Kit | BD-2 clia kit

BD-2 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510757

• Gene Names: DEFB4B; DEFB4P
• Reactivity: Human
• Synonyms: BD-2; BD-2 (Human) LumiAb ELISA Kit; Human BD-2; hBD-2; Defensin beta 2; Skin-antimicrobial peptide 1; SAP1; DEFB4; DEFB102; DEFB2; DEFB4P; Beta-defensin 2; BD-2 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human 4-1BBR ELISA is capable of recognizing both recombinant and naturally produced Human 4-1BBR proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 64

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BD-2 clia kit

Principle of the Assay: The LumiAb Human 4-1BB-R Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human 4-1BB-R concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human 4-1BB-R while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human 4-1BB Ligand Receptor, also known as the Tumor Necrosis Factor Receptor Superfamily Member 9, is a 255 amino acid cytokine protein implicated in T-cell activation pathways. After initial synthesis of the peptide, the cytokine is proteolytically cleaved by proteases, thus separating the 23 residue signal sequence from the actual mature 232 residue 4-1BB Receptor sequence. The protein is expressed from the TNFRSF9 gene located at locus 1p36 on chromosome 1. The 4-1BB receptor or TNFRSF9 inherently has high affinity binding for the 4-1BB ligand such that upon binding, the receptor contributes to clonal expansion, survival and development of T-cells. It can also induce proliferation in peripheral monocytes, enhance T-cell apoptosis induced by TCR/CD3 triggered activation and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins such as TRAF1, TRAF2 and TRAF 3 have been shown to bind to this receptor and transducer the signals leading to activation of NF-kappaB.

Product Categories/Family for BD-2 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 3023383
• NCBI GeneID: 100289462
• UniProt Accession #: O15263
• NCBI Official Full Name: Beta-defensin 4A
• NCBI Official Synonym Full Names: defensin beta 4B
• NCBI Official Symbol: DEFB4B
• NCBI Official Synonym Symbols: DEFB4P
• NCBI Protein Information: beta-defensin 4B
• UniProt Protein Name: Beta-defensin 4A
• UniProt Gene Name: DEFB4A
• UniProt Synonym Gene Names: DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
• UniProt Entry Name: DFB4A_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to bacterium; innate immune response; immune response; chemotaxis