Human PLGF CLIA Kit | PLGF clia kit

PLGF (Human) LumiAb ELISA Kit

Cat. Num. AAA9510811

• Gene Names: PGF; PGFL; PIGF; PLGF; PlGF-2; D12S1900; SHGC-10760
• Reactivity: Human
• Synonyms: PLGF; PLGF (Human) LumiAb ELISA Kit; Human PLGF; Placenta growth factor; PGFL; PLGF clia kit

• Reactivity: Human
• Specificity: The LumiAb Human NOV ELISA is capable of recognizing both recombinant and naturally produced Human NOV proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 149

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLGF clia kit

Principle of the Assay: The LumiAb Human NOV Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human NOV concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human NOV while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human NOV or Nephroblastoma-Overexpressed Gene, is a 357 amino acid, cysteine-rich family member of the CCN family of regulatory proteins encoded by the NOV gene located at locus 8q24.1 on chromosome 8. After initial synthesis of the protein, it is proteolytically cleaved to remove the 31 residue signal sequence, allowing the 326 residue peptide to fold properly into a mature NOV cytokine. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. Moreover, belonging to the CCN family, NOV contains 1 CTCK (C-terminal cysteine knot-like) domain, 1 IGFBP N-terminal domain, 1 TSP type-1 domain and a VWFC domain. NOV is typically expressed in bone marrow, thymic cells and nephroblastoma with increased expression in Wilms tumor of the stromal type and down-regulated expression by WT1.

Product Categories/Family for PLGF clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 333033785
• NCBI GeneID: 5228
• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763
• NCBI Official Full Name: placenta growth factor isoform 2
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: PGF
• NCBI Official Synonym Symbols: PGFL; PIGF; PLGF; PlGF-2; D12S1900; SHGC-10760
• NCBI Protein Information: placenta growth factor
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: PGF
• UniProt Synonym Gene Names: PGFL; PLGF; PlGF
• UniProt Entry Name: PLGF_HUMAN

NCBI Description

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity

Biological Process: response to drug; organ regeneration; female pregnancy; signal transduction; cellular response to hormone stimulus; positive regulation of angiogenesis; cell-cell signaling; ureteric bud branching; positive regulation of cell division; positive regulation of cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation