Human CD163 CLIA Kit | CD163 clia kit

CD163 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510961

• Gene Names: CD163; M130; MM130; SCARI1
• Reactivity: Human
• Synonyms: CD163; CD163 (Human) LumiAb ELISA Kit; Human CD163; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; M130; CD163 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Carbonic Anhydrase IX ELISA is capable of recognizing both recombinant and naturally produced Human Carbonic Anhydrase IX proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Sequence Length: 1156

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD163 clia kit

Principle of the Assay: The LumiAb Human Carbonic Anhydrase IX Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Carbonic Anhydrase IX concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Carbonic Anhydrase IX while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Carbonic Anhydrase IX is a 459 amino acid protein that forms oligomers linked by disulfide bonds. Asn-346 bears high-mannose type glycan structures after post-translational modification. Carbonic Anhydrase IX is induced by hypoxia and is inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). It forms oligomers linked by disulfide bonds and is found on the surface microvilli and in the nucleus, particularly in the nucleolus. Carbonic Anhydrase IX is a single-pass type I membrane protein that belongs to the alpha-carbonic anhydrase family and is expressed primarily in carcinoma cell lines. It is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Carbonic Anhydrase IX catalyzes reversible hydration of carbon dioxide and participates in pH regulation. It may be involved in the control of cell proliferation and transformation, and it appears to be a novel specific biomarker for a cervical neoplasia.

Product Categories/Family for CD163 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 344179110
• NCBI GeneID: 9332
• NCBI Accession #: NP_004235.4
• NCBI GenBank Nucleotide #: NM_004244.5
• UniProt Accession #: Q86VB7
• NCBI Official Full Name: scavenger receptor cysteine-rich type 1 protein M130 isoform a
• NCBI Official Synonym Full Names: CD163 molecule
• NCBI Official Symbol: CD163
• NCBI Official Synonym Symbols: M130; MM130; SCARI1
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Protein Family: Scavenger receptor cysteine-rich type 1 protein
• UniProt Gene Name: CD163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response