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Testing Data

General Phosphatidylglycerol Assay Kit

Phosphatidylglycerol Assay Kit (Fluorometric)

Reactivity
General
Synonyms
Phosphatidylglycerol; Phosphatidylglycerol Assay Kit (Fluorometric); glycerophospholipid; Phosphatidylglycerol assay kit
Ordering
For Research Use Only!
Reactivity
General
Samples
Lipid extracts from tissue and cell lysates
Lipid extracts from variousbiological fluids (e.g.serum, plasma)
Detection Method
Fluorometric: 535/587nm
Features & Benefits
The first kit in the market that detects Phosphatidylglycerol levels in biological samples
Can be completed in less than one hour
Specific
validated with biological samples
Simple
easy protocols
Measurement of PG content of various tissue/cell extracts Determination of PG concentration in biological fluids
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

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Related Product Information for Phosphatidylglycerol assay kit
Phospholipids are physiologically critical amphiphilic molecules that are involved in regulation of numerous cellular processes. Phosphatidylglycerol (PG) is a glycerophospholipid consisting of a glycerol backbone with two acyl chains and a phosphatidyl group that carries another glycerol moiety. Lung surfactant is an important lipid-protein mixture that aids in expansion and prevents total collapse of the lung during respiration. PG is a prominent phospholipid in lung surfactant, and its presence can be monitored as a measure of lung development in the maturing fetus. In addition, most bacteria utilize PG in their membrane, and plants utilize this component as the major phospholipid in the photosynthetic membranes of leaf tissue. In HPLC analysis of phospholipids, phosphatidic acid can interfere with and complicate determination of PG content. Phosphatidylglycerol Assay Kit utilizes specific enzymes to generate an intermediate that reacts with a probe, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PG present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids (see figure below) and capable of detecting as little as 20 pmoles of phosphatidylglycerol.

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Product Notes

The General Phosphatidylglycerol (Catalog #AAA849702) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849702 Assay Kit recognizes General Phosphatidylglycerol. It is sometimes possible for the material contained within the vial of "Phosphatidylglycerol, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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