Rabbit POLR1C Polyclonal Antibody | anti-POLR1C antibody

POLR1C Polyclonal Antibody

Cat. Num. AAA3006189

• Gene Names: POLR1C; AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR1C; Polyclonal Antibody; POLR1C Polyclonal Antibody; DNA-directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III; subunit AC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40; POLR1E; anti-POLR1C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR1C polyclonal antibody detects endogenous levels of POLR1C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 346

• Applicable Applications for anti-POLR1C antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human POLR1C.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POLR1C polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:NIH-3T3 cell lysate)

Related Product Information for anti-POLR1C antibody

RNA polymerases transcribe nuclear genes for ribosomal RNA, thus representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I transcription. RPA16, RPA40 and RPA135 are subunits of Pol I that associate with each other at an early stage of RNA Pol I assembly. RPA40 is essential for the function and integrity of the complex and is also an essential subunit of RNA polymerase III (Pol III).

NCBI and Uniprot Product Information

• NCBI GI #: 42560246
• NCBI GeneID: 9533
• NCBI Accession #: NP_976035.1
• NCBI GenBank Nucleotide #: NP_976035.1
• UniProt Accession #: O15160
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: DNA-directed RNA polymerases I and III subunit RPAC1 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase I and III subunit C
• NCBI Official Symbol: POLR1C
• NCBI Official Synonym Symbols: AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• NCBI Protein Information: DNA-directed RNA polymerases I and III subunit RPAC1
• UniProt Protein Name: DNA-directed RNA polymerases I and III subunit RPAC1
• Protein Family: DNA-directed RNA polymerases
• UniProt Gene Name: POLR1C
• UniProt Synonym Gene Names: POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40
• UniProt Entry Name: RPAC1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 2.7.7.6; Transferase

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; innate immune response; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3