Testing Data
(PHA stimulated human PBMCs stained with Mouse anti Human CD69 (MBS224552) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Mouse CD69 Monoclonal Antibody | anti-CD69 antibody
MOUSE ANTI HUMAN CD69:FITC
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
CD69; Monoclonal Antibody; MOUSE ANTI HUMAN CD69:FITC; anti-CD69 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FN50
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
199
Applicable Applications for anti-CD69 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(PHA stimulated human PBMCs stained with Mouse anti Human CD69 (MBS224552) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Testing Data
(PHA stimulated human PBMCs stained with Mouse anti Human CD69 (MBS224552) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Testing Data
(PHA stimulated human PBMCs stained with Mouse anti Human CD69:FITC)
Testing Data
(PHA stimulated human PBMCs stained with Mouse anti Human CD69:FITC)
Related Product Information for anti-CD69 antibody
Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60kDa (López-Cabrera et al. 1993). CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,559 Da
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69; C-type lectin domain family 2, member C; CD69 antigen (p60, early T-cell activation antigen); activation inducer molecule (AIM/CD69); early T-cell activation antigen p60; early lymphocyte activation antigen; leukocyte surface antigen Leu-23
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
NCBI Description
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Uniprot Description
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Research Articles on CD69
Similar Products
Product Notes
The CD69 cd69 (Catalog #AAA214557) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
