Rabbit OAZ3 Polyclonal Antibody | anti-OAZ3 antibody

OAZ3 Polyclonal Antibody

Cat. Num. AAA3006453

• Gene Names: OAZ3; AZ3; OAZ-t; TISP15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: OAZ3; Polyclonal Antibody; OAZ3 Polyclonal Antibody; Ornithine decarboxylase antizyme 3; AZ3; ODC-Az 3; anti-OAZ3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OAZ3 polyclonal antibody detects endogenous levels of OAZ3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 190

• Applicable Applications for anti-OAZ3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human OAZ3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of OAZ3 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Mouse testis tissue lysateLane3:Rat testis tissue lysate)

Related Product Information for anti-OAZ3 antibody

The antizyme family is a group of small proteins that play a role in cell growth and division by regulating the biosynthesis of polyamines such as putrescine, spermidine and spermine. AZ3, also known as OAZ3 (Ornithine decarboxylase antizyme 3), is a 187 amino acid protein that belongs to the ODC antizyme family. AZ3 probably plays a key role in spermatogenesis by regulating the intracellular concentration of polyamines in haploid germ cells because it binds to, and destabilizes, ornithine decarboxylase. However, mutations in the AZ3 gene are not a common cause of male infertility and the normal AZ3 protein does not accelerate ornithine decarboxylase degeneration. AZ3 expression has a sharp onset in early spermatids, peaks in later stages and is gone in spermatozoa.

NCBI and Uniprot Product Information

• NCBI GI #: 201023343
• NCBI GeneID: 51686
• NCBI Accession #: NP_001128411.1
• NCBI GenBank Nucleotide #: NP_001128411.1
• UniProt Accession #: Q9UMX2
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: ornithine decarboxylase antizyme 3 isoform 2
• NCBI Official Synonym Full Names: ornithine decarboxylase antizyme 3
• NCBI Official Symbol: OAZ3
• NCBI Official Synonym Symbols: AZ3; OAZ-t; TISP15
• NCBI Protein Information: ornithine decarboxylase antizyme 3
• UniProt Protein Name: Ornithine decarboxylase antizyme 3
• Protein Family: Ornithine decarboxylase antizyme
• UniProt Gene Name: OAZ3
• UniProt Synonym Gene Names: AZ3; ODC-Az 3
• UniProt Entry Name: OAZ3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake; however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. Alternatively spliced transcript variants encoding different isoforms, including one resulting from the use of non-AUG (CUG) translation initiation codon, have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

OAZ3: Binds to, and destabilizes, ornithine decarboxylase. Does not accelerate ornithine decarboxylase degeneration. OAZ3 probably plays a key role in spermatogenesis by regulating the intracellular concentration of polyamines in haploid germ cells. Belongs to the ODC antizyme family. 1 isoforms of the human protein are produced by ribosomal frameshifting.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; putrescine transmembrane transporter activity; ornithine decarboxylase inhibitor activity

Biological Process: polyamine biosynthetic process; negative regulation of catalytic activity; positive regulation of protein catabolic process; spermatogenesis; regulation of phosphoprotein phosphatase activity; regulation of amino acid metabolic process; putrescine transport