gag gene recombinant protein

gag gene recombinant

Cat. Num. AAA505036

• Applications: ELISA, Western Blot
• Purity: 0.98
• Synonyms: gag gene; gag gene recombinant; RECOMBINANT ANTIGEN p24 from HIV; p24 protein; gag gene recombinant protein

• Host: E Coli
• Purity/Purification: 0.98
• Form/Format: Dry powder (lyophilized)
• Concentration: 0.98 mg/ml (varies by lot)
• Sequence Length: 212

• Applicable Applications for gag gene recombinant protein: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended concentration in ELISA plates: 0.65 ug/ml in plates for IgG detection; Suggested Titer by ELISA: 1:1500, which corresponds to 0.65 g/ml in plates, for IgG detection.
• Recombinant Antigen: human immunodeficiency virus core antigen p24 (Sundqvist et al., 1989).
• Organism: HIV
• Tag: HIS
• Molecular Weight Information: Determined by SDS-PAGE, the protein band is between molecular markers of 35,000-25,000 Da, while relative molecular mass calculated from amino acid sequence is 30,065.0 Da.
• Observation Notes: in some cases, purified proteins runat a molecular weight which is slightly different to thetheoretically calculated molecular weight maybe due to the his-tag existence, which can produce a delay in SDS-PAGE.Proteins should be maintained frozen at highconcentrations. The dilution to be performed for ELISAassays should be made with a small quantity of protein, thesame day of the experiment. In order to defrost the protein,maintain the aliquot at 25 degree C without shaking to avoidaggregation. Prior making test dilutions and after defrostthe protein, is recommended to remove possible proteinaggregates by centrifuging the stock solution, avoidingalterations in the immobilization of the biomolecule to thesolid surface.
• Buffer: 20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 250 mM Imidazol
• Batch Composition: Recombinant antigen with a his-tag in its N-terminus
• Storage Buffer: 20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 250 mM Imidazol
• Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
• Preparation and Storage: Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Testing Data

Testing Data #2

Related Product Information for gag gene recombinant protein

the gag gene for the core antigen p24 hasbeen expressed as a recombinant antigen fused to a his-tagin its N-terminus. It is produced from the complete ORFs ofthis gene, aa (1-212).

Product Categories/Family for gag gene recombinant protein

Viruses

References

Sundqvist VA, Albert J, Ohlsson E, Hinkula J, FenyöEM, Wahren B. Human immunodeficiency virus type 1 p24production and antigenic variation in tissue culture ofisolates with various growth characteristics. 1989, J MedVirol, 29(3):170-5.
Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.

NCBI and Uniprot Product Information

• NCBI GI #: 2181212
• UniProt Accession #: O11943
• Molecular Weight: 23,597 Da
• NCBI Official Full Name: p24 protein
• UniProt Protein Name: P24 protein
• UniProt Gene Name: gag
• UniProt Entry Name: O11943_9HIV1