Rabbit POLR3B Polyclonal Antibody | anti-POLR3B antibody

POLR3B Polyclonal Antibody

Cat. Num. AAA3007600

• Gene Names: POLR3B; C128; HLD8; RPC2; INMAP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR3B; Polyclonal Antibody; POLR3B Polyclonal Antibody; DNA-directed RNA polymerase III subunit RPC2; RNA polymerase III subunit C2; C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; anti-POLR3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR3B polyclonal antibody detects endogenous levels of POLR3B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1075

• Applicable Applications for anti-POLR3B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 330-380 of Human POLR3B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POLR3B polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate (20ug) Lane2:H9C2 whole cell lysate (20ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-POLR3B antibody

RNA polymerase II (Pol II) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3B (polymerase (RNA) III (DNA directed) polypeptide B), also known as C128 or RPC2, is a 1, 133 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the second largest component of the multi-protein RNA polymerase III (Pol III) complex, POLR3B functions as a DNA-dependent RNA polymerase that specifically catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. The gene encoding POLR3B maps to human chromosome 12, which encodes over 1, 100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 238908505
• NCBI GeneID: 55703
• NCBI Accession #: NP_001154180.1
• NCBI GenBank Nucleotide #: NP_001154180.1
• UniProt Accession #: Q9NW08
• Molecular Weight: ~ 127kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC2 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit B
• NCBI Official Symbol: POLR3B
• NCBI Official Synonym Symbols: C128; HLD8; RPC2; INMAP
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC2
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC2
• UniProt Gene Name: POLR3B
• UniProt Synonym Gene Names: RNA polymerase III subunit C2
• UniProt Entry Name: RPC2_HUMAN

NCBI Description

This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]