Testing Data
p14 recombinant protein
p14 recombinant
Applications
ELISA, Western Blot, Lateral Flow
Purity
0.95
Synonyms
p14; p14 recombinant; RECOMBINANT ANTIGEN p14 from Borrelia afzelii; flagelina p41 of B. afzelii; p14 recombinant protein
Host
E Coli
Purity/Purification
0.95
Form/Format
Dry powder (lyophilized)
Concentration
1.522 mg/ml (varies by lot)
Sequence Length
160
Applicable Applications for p14 recombinant protein
ELISA (EIA), Western Blot (WB), Lateral Flow (LF)
Application Notes
Recommended concentration in ELISA plates: 0.9 ug/ml in plates for IgG detection
Suggested Titer by ELISA: approx. 1/1750, which corresponds to 0.9 g/ml in plates for IgG detection.
Suggested Titer by ELISA: approx. 1/1750, which corresponds to 0.9 g/ml in plates for IgG detection.
Recombinant Antigen
internal 14-kDa flagellin fragment of Borrelia afzelii (Craft et al., 1984).
Organism
Borrelia
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, the protein band is between molecular markers of 25,000 and18,400 Da, while relative molecular mass calculated from amino acid sequence is 16,483.9 Da
Observation Notes
in some cases, purified proteins runat a molecular weight which is slightly different to thetheoretically calculated molecular weight maybe due to thehis-tag presence, which can produce a delay in SDS-PAGE.Proteins should be maintained frozen at highconcentrations. The dilution to be performed for ELISAassays should be made with a small quantity of protein, thesame day of the experiment. In order to defrost the protein,maintain the aliquot at 25 degree C without shaking to avoidaggregation. Prior making test dilutions and after defrostthe protein, is recommended to remove possible proteinaggregates by centrifuging the stock solution, avoidingalterations in the immobilization of the biomolecule to thesolid surface.
Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 8 M urea and 250 mM imidazol
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 8 M urea and 250 mM imidazol
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Testing Data #3
Testing Data #3
Related Product Information for p14 recombinant protein
the Borrelia afzelii antigen p14 has been prepared as a recombinant antigen fused to a his-tag in its N-terminus. It is produced from the internal fragment (aa 197-274) of the 41 kDa-flagellin of this bacteria.
Product Categories/Family for p14 recombinant protein
References
Craft, J. E., D. K. Fischer, J. A. Hardin, M. Garcia- Blanco, and A. C. Steere. Spirochetal antigens in Lymedisease. 1984, Arthritis Rheum. 27(Suppl.):64.
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.Important
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.Important
NCBI and Uniprot Product Information
Similar Products
Product Notes
The p14 (Catalog #AAA505001) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Lateral Flow (LF). Recommended concentration in ELISA plates: 0.9 ug/ml in plates for IgG detection Suggested Titer by ELISA: approx. 1/1750, which corresponds to 0.9 g/ml in plates for IgG detection. Researchers should empirically determine the suitability of the p14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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