Quality Control
OMP recombinant protein
Recombinant antigen OMP for Salmonella typhi
Applications
ELISA
Purity
>90% in a SDS-PAGE
Synonyms
OMP; Recombinant antigen OMP for Salmonella typhi; OMP recombinant protein
Purity/Purification
>90% in a SDS-PAGE
Form/Format
Dry powder (lyophilized)
Sequence Length
136
Applicable Applications for OMP recombinant protein
ELISA
Application Notes
ELISA: 1:1,200, which-corresponds to 1 f.g/ ml of protein concentration in plates for IgG detection.
Source
Escherichia coli
Recombinant Antigen
outer membrane channel protein TolC (OMP50) of Salmonella typhi (Parkhill et al., 2001)
Molecular Weight
determined by SDS-PAGE, the protein band is between molecular markers of 35,000- 25,000 Da, while relative molecular mass calculated from amino acid sequence is 32,080.30 Da .
his-OMP
recombinant antigen with a his-tag in its N-terminus
Total Quantity Per Aliquot
0.5 mg
Observation
proteins should be maintained frozen at high concentrations. The dilution to be performed for EUSA assays should be made with a small quantity of protein, the same day of the experiment. Once reconstituted and in order to defrost the protein, maintain the aliquot at 2SOC without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Quality Control
Quality Control
Related Product Information for OMP recombinant protein
some of the antigeniC regions of Salmonella typhi antigen OMP has been prepared as a recombinant antigen fused to a his-tag in its N-terminus.
Product Categories/Family for OMP recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
15,896 Da
NCBI Official Full Name
OMP, partial
UniProt Protein Name
OMP
Protein Family
UniProt Gene Name
OMP
Similar Products
Product Notes
The OMP omp (Catalog #AAA505076) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's OMP can be used in a range of immunoassay formats including, but not limited to, ELISA. ELISA: 1:1,200, which-corresponds to 1 f.g/ ml of protein concentration in plates for IgG detection. Researchers should empirically determine the suitability of the OMP omp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OMP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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