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Testing Data
pp52 Recombinant Protein | UL44 recombinant protein
RECOMBINANT ANTIGEN pp52 from Cytomegalovirus (CMV)
Applications
ELISA, Western Blot
Synonyms
pp52; RECOMBINANT ANTIGEN pp52 from Cytomegalovirus (CMV); UL44 recombinant protein
Host
E Coli
Form/Format
Dry powder (lyophilized)
Concentration
1.32 mg/ml (varies by lot)
Sequence Length
119
Applicable Applications for UL44 recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA and Western blot assays. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.
Suggested Titer By Elisa
Up to 1/1,760, which corresponds to 0.75 ug/ml of protein concentration in plates for IgM and IgG detection
Suggested Titer By Elisa
Up to 1/1,760, which corresponds to 0.75 ug/ml of protein concentration in plates for IgM and IgG detection
Observations
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Organism
CMV
Antigen
pp52-truncated
Recombinant Antigen
CMV phosphoprotein of 52 kDa (Maine et al., 2000).
Reconstitution
With approx. 0.66 ml of sterile double-distilled water for a final concentration of approx. 1.32 mg/ml. The protein will be at pH8.
Batch Composition
Componets: his-pp52
Composition: recombinant antigen with his-tag in its N-terminus
Composition: recombinant antigen with his-tag in its N-terminus
Preparation and Storage
Protein is shipped at room temperature. Upon arrival, it should be stored at 4°C or -20°C. Once reconstituted, it should aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
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Related Product Information for UL44 recombinant protein
The CMV antigen pp52 has been prepared as a truncated recombinant antigen fused to a his-tag in its N-terminal. It is produced from the UL44 gene which codifies the DNA polymerase processivity subunit of CMV.
Product Categories/Family for UL44 recombinant protein
References
Maine, G.T., R. Stricker, M. Schuler, J. Spesard, S. Brojanac, B. Iriarte, K. Herwig, T. Gramins, B. Combs, J. Wise, H. Simmons, T. Gram, J. Lonze, D. Ruzicki, B. Byrne, J. D. Clifton, L. E. Chovan, D. Wachta, C. Holas, D. Wang, T. Wilson, S. Tomazic-Allen, M. A. Clements, G. L. Wright, Jr., T. Lazzarotto, A. Ripalti, andM. P. Landini. Development and Clinical Evaluation of a Recombinant-Antigen-Based Cytomegalovirus Immunoglobulin M Automated Immunoassay Using the Abbott AxSYM Analyzer. 2000, J Clin Microbiol., 38(4):1476-1481.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
Determined by SDS-PAGE, the protein band is between molecular markers of 14,400 Da, while relative molecular mass calculated from amino acid sequence is 11,251.1 Da.
NCBI Official Full Name
PP52
UniProt Protein Name
PP52
UniProt Entry Name
F1AXA5_ORFV
Similar Products
Product Notes
The UL44 (Catalog #AAA505056) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's pp52 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA and Western blot assays. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. Suggested Titer By Elisa Up to 1/1,760, which corresponds to 0.75 ug/ml of protein concentration in plates for IgM and IgG detection. Researchers should empirically determine the suitability of the UL44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "pp52, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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