Testing Data
OspC recombinant protein
OspC recombinant
Purity
0.95
Synonyms
OspC; OspC recombinant; RECOMBINANT ANTIGEN ospC from Borrelia afzelii; B. afzelii; OspC recombinant protein
Host
E Coli
Purity/Purification
0.95
Form/Format
Dry powder (lyophilized)
Concentration
0.908 mg/ml (varies by lot)
Sequence Length
212
Recombinant Antigen
the outer surface protein C (OspC) of Borrelia afzelii (Rousselle et al., 1998).
Organism
Borrelia
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, theprotein band is between molecular markers of 25,000 and35,000 Da, while relative molecular mass calculated fromamino acid sequence is 31,320.3 Da.
Observation Notes
in some cases, purified proteins runat a molecular weight which is slightly different to thetheoretically calculated molecular weight maybe due to thehis-tag presence, which can produce a delay in SDS-PAGE.Proteins should be maintained frozen at highconcentrations. The dilution to be performed for ELISAassays should be made with a small quantity of protein, thesame day of the experiment. In order to defrost the protein,maintain the aliquot at 25 degree C without shaking to avoidaggregation. Prior making test dilutions and after defrostthe protein, is recommended to remove possible proteinaggregates by centrifuging the stock solution, avoidingalterations in the immobilization of the biomolecule to thesolid surface.
Buffer
20 mM phosphate buffer pH 8, 1 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether, urea 8 M
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 1 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether, urea 8 M
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for OspC recombinant protein
the Borrelia afzelii antigen OspC has been prepared as a recombinant antigen fused to a his-tag in its N-terminus. It is produced from the complete ORF of the gene of the outer surface protein C.
Product Categories/Family for OspC recombinant protein
References
Rousselle J. C., Callister S. M., Schell R. F., Lovrich S.D., Jobe D. A., Marks J. A. and Wieneke C. A.Borreliacidal antibody production against outer surfaceprotein C of Borrelia burgdorferi. 1998, J. Infect. Dis.,178(3):733-41.
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding. Anal Biochem. 1976,131:499-503.
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding. Anal Biochem. 1976,131:499-503.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
outer surface protein C
NCBI Official Symbol
ospC
NCBI Protein Information
outer surface protein C
Protein Family
Research Articles on OspC
Similar Products
Product Notes
The OspC (Catalog #AAA505002) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OspC, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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