BALF2 recombinant protein

BALF2 recombinant

Cat. Num. AAA505010

• Applications: ELISA, Western Blot
• Purity: 0.95
• Synonyms: BALF2; BALF2 recombinant; RECOMBINANT ANTIGEN p138 from Epstein-Barr Virus (EBV); p138 (D-EA); BALF2 recombinant protein

• Host: E Coli
• Purity/Purification: 0.95
• Form/Format: Dry powder (lyophilized)
• Concentration: 1.59 mg/ml (varies by lot)
• Sequence Length: 1128

• Applicable Applications for BALF2 recombinant protein: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended concentration in ELISA plates: 2 ug/ml in plates for IgM detection
• Recombinant Antigen: EBV antigen p138 (Mumtsidu et al., 2008).
• Organism: EBV
• Tag: HIS
• Molecular Weight Information: Determined by SDS-PAGE, the protein band is between molecular markers of 66,200-116,000 Da, while relative molecular mass calculated from amino acid sequence is 129,700.0 Da.
• Suggested Titer by ELISA: approx. 1:3250, which corresponds to 0.49ug/ml of protein concentration in plates for IgG detection.
• Observation Notes: Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
• Buffer: 20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
• Batch Composition: Recombinant antigen with a his-tag in its N-terminus
• Storage Buffer: 20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
• Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
• Preparation and Storage: Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Testing Data

Testing Data #2

Related Product Information for BALF2 recombinant protein

The early antigen p138 has been prepared as a recombinant antigen fused to a his-tag. It is produced from the complete ORF of the BALF2 gene which codifies the major DNA binding protein of EBV.

Product Categories/Family for BALF2 recombinant protein

Viruses

References

Mumtsidu E, Makhov AM, Konarev PV, Svergun DI, Griffith JD, Tucker PA. Structural features of the single-stranded DNA-binding protein of Epstein-Barr virus. 2008 J. Struct. Biol. 161(2):172-87.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

NCBI and Uniprot Product Information

• NCBI GI #: 82503273
• NCBI GeneID: 3783678
• NCBI Accession #: YP_401717.1
• NCBI GenBank Nucleotide #: NC_007605.1
• UniProt Accession #: P03227; Q777A7
• Molecular Weight: 123,122 Da
• NCBI Official Full Name: BALF2
• NCBI Official Symbol: BALF2
• NCBI Protein Information: single-stranded DNA binding protein
• UniProt Protein Name: Major DNA-binding protein
• UniProt Entry Name: DNBI_EBVB9

Uniprot Description

Function: Plays a role in virus genomic DNA replication associated with the viral DNA polymerase complex. Destabilizes double-stranded DNA in an ATP independent manner. May disrupt loops, hairpins and other secondary structures present on ssDNA by binding directly to it, thereby reducing and eliminating pausing of the viral DNA polymerase at specific sites. May participate in the opening of the lytic phase origin (ori-Lyt) region of the viral DNA to initiate viral replication. Ref.2

Subunit structure: Forms a complex with the DNA polymerase BALF5, the DNA polymerase processivity factor BMRF1, and the alkaline exonuclease BGLF5.

Subcellular location: Virion tegument. Host nucleus. Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs. Ref.4 Ref.5

Sequence similarities: Belongs to the herpesviridae DNA-binding protein family.