1F8 recombinant protein

1F8 recombinant

Cat. Num. AAA505020

• Applications: ELISA, Western Blot
• Purity: 0.98
• Synonyms: 1F8; 1F8 recombinant; RECOMBINANT ANTIGEN E from West Nile Virus; FcaBP; 1F8 recombinant protein

• Host: E Coli
• Purity/Purification: 0.98
• Form/Format: Dry powder (lyophilized)
• Concentration: 1.24 mg/mL (varies by lot)
• Sequence Length: 3433

• Applicable Applications for 1F8 recombinant protein: ELISA (EIA), Western Blot (WB)
• Application Notes: SUGGESTED TITER BY ELISA: 1:1200, this corresponds to 1.00 ug/ml of protein concentration in plates for IgG and IgM detection.; ; ; ELISA and Western blot assays. Wherethis product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.
• Total Volume per Aliquot: 0.084 mL
• Recombinant Antigen: West Nile virus antigen E (Beasley et al., 2004).
• Organism: Trypanosoma cruzi
• Tag: HIS/GST
• MW Note: Determined by SDS-PAGE, the protein band is between molecular markers of 45,000-66,200 Da, while relative molecular mass calculated from amino acid sequence is 56,838.8Da.
• Lot Composition: Components: GST-his-1F8; ; Composition: Recombinant antigen with a with A GST-TAG and a his-tag in its N-terminus.
• Buffer: 20 Mm phosphate buffer pH 8.0, 1 M NaCI
• Quality Control: 1. PROTEIN CONCENTRATION DETERMINED ESPECTROPHOTOMETRICALLY; ; DO₂₈₀=1.44; A _{0.1 %}(=1 g/l)= 1.158; CONCENTRATION*:1.24 mg/ml; ; *The measurement of the protein concentration has been performed with the theoretical xtinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989
• Observation Notes: Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
• Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
• Preparation and Storage: Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Testing Data

Testing Data

Testing Data

Related Product Information for 1F8 recombinant protein

The T. cruzi antigen 1F8 has been prepared as a recombinant antigen fused to a GST - his-tag in its N-terminal. It is produced from the 1F8 gene of the T. cruzi calcium-binding protein.

Product Categories/Family for 1F8 recombinant protein

PARASITES

References

González, A., T. J., Lerner, M. Huecas, B. Sisa-Pineda, N. Nogueira, and P. M. Lizardi. Apparent generation of a segmented mRNA from two separate tándem gene families in Trypanosoma cruzi. 1985, Nucleic Acids Res. 13:5789-5803.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

NCBI and Uniprot Product Information

• NCBI GI #: 377814031
• NCBI Official Full Name: polyprotein