Testing Data
FRA recombinant protein
FRA recombinant
Applications
ELISA, Western Blot
Purity
0.98
Synonyms
FRA; FRA recombinant; RECOMBINANT ANTIGEN FRA from Trypanosoma cruzi; 2 repetitions from citoskeleton-associated protein; FRA recombinant protein
Host
E Coli
Purity/Purification
0.98
Form/Format
Dry powder (lyophilized)
Concentration
0.75 mg/ml (varies by lot)
Sequence Length
326
Applicable Applications for FRA recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended concentration in ELISA plates: 1 ug/ml in plates for IgG detection
Suggested Titer by ELISA: 1:750, which corresponds to 1 g/ml in plates for IgG detection
Suggested Titer by ELISA: 1:750, which corresponds to 1 g/ml in plates for IgG detection
Recombinant Antigen
Trypanosoma cruzi antigen FRA (Foti et al., 2009).
Organism
Trypanosoma cruzi
Tag
HIS/GST
Molecular Weight Information
Determined by SDS-PAGE, theprotein band is between molecular markers of 45,000-66,200 Da, while relative molecular mass calculated fromamino acid sequence is 49,832.5 Da.
Observation Notes
Proteins should be maintained frozenat high concentrations. The dilution to be performed forELISA assays should be made with a small quantity ofprotein, the same day of the experiment. In order to defrostthe protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface.
Buffer
50 mM Tris-HCl pH 8 and 10 mM glutathion
Batch Composition
Recombinant antigen with a GST-tag and a his-tag in its N-terminus
Storage Buffer
50 mM Tris-HCl pH 8 and 10 mM glutathion
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for FRA recombinant protein
the T. cruzi antigen FRA has beenprepared as a recombinant antigen fused to a GST-his-tagin its N-terminal. It consists of two consecutive repetitionsof the characteristic 64 aa-repetitions of the T. cruzicytoskeleton assocociated protein.
Product Categories/Family for FRA recombinant protein
References
Foti, L., Fonseca B. de P., Nascimiento, L. D., MarquesC. de F., da Silva E. D., Duarte, C. A., Probst C. M.,Goldenberg, S., Pinto, A. G. and Krieger, M. A. Viabilitysuty of a multiplex diagnostic platform for Chagas diseases.2009, Mems.Instituto Oswaldo Cruz, 104:136-41.
Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.
Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The FRA (Catalog #AAA505051) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FRA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended concentration in ELISA plates: 1 ug/ml in plates for IgG detection Suggested Titer by ELISA: 1:750, which corresponds to 1 g/ml in plates for IgG detection. Researchers should empirically determine the suitability of the FRA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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