Rabbit anti-Human, Mouse Transcription Factor E3 Polyclonal Antibody | anti-TFE3 antibody

Transcription Factor E3 (TFE3, Transcription Factor Binding to IGHM Enhancer 3, Class E Basic Helix-loop-helix Protein 33, bHLHe33, RCCP2, TFEA) (AP)

Cat. Num. AAA6397083

• Gene Names: TFE3; TFEA; RCCP2; RCCX1; bHLHe33
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Transcription Factor E3; Polyclonal Antibody; Transcription Factor E3 (TFE3; Transcription Factor Binding to IGHM Enhancer 3; Class E Basic Helix-loop-helix Protein 33; bHLHe33; RCCP2; TFEA) (AP); anti-TFE3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TFE3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TFE3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TFE3, aa1-575 (NP_006512.2).
• Immunogen Sequence: MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TFE3 rabbit polyclonal antibody. Western Blot analysis of TFE3 expression in mouse kidney.)

Western Blot (WB)

(Western Blot analysis of TFE3 expression in transfected 293T cell line by TFE3 polyclonal antibody. Lane 1: TFE3 transfected lysate (61.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TFE3 antibody

The DNA-binding factor TFE-3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. TFE-3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE-3 binds and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination.

Product Categories/Family for anti-TFE3 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 8659574
• NCBI GeneID: 7030
• NCBI Accession #: NP_006512.2
• NCBI GenBank Nucleotide #: NM_006521.3
• UniProt Accession #: P19532; Q92757; Q92758; Q99964; A8MZL6
• Molecular Weight: 61,521 Da
• NCBI Official Full Name: transcription factor E3
• NCBI Official Synonym Full Names: transcription factor binding to IGHM enhancer 3
• NCBI Official Symbol: TFE3
• NCBI Official Synonym Symbols: TFEA; RCCP2; RCCX1; bHLHe33
• NCBI Protein Information: transcription factor E3; Transcription factor for IgH enhancer; transcription factor E family, member A; class E basic helix-loop-helix protein 33
• UniProt Protein Name: Transcription factor E3
• Protein Family: Transcription factor
• UniProt Gene Name: TFE3
• UniProt Synonym Gene Names: BHLHE33; bHLHe33
• UniProt Entry Name: TFE3_HUMAN

NCBI Description

The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.[provided by RefSeq, Dec 2009]

Uniprot Description

TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: regulation of osteoclast differentiation; positive regulation of cell adhesion; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; humoral immune response

Disease: Renal Cell Carcinoma, Xp11-associated