ELISA
(Titration Curve by an ELISA Assay. The titer has been suggested in reference to an "in-house" ELISA kit performed at the lab over the first lot obtained. Each end user should carry out their own titration for their particular application. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgG sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera. )
BZLF1 recombinant protein
BZLF1 recombinant
Applications
ELISA, Western Blot
Synonyms
BZLF1; BZLF1 recombinant; Zebra (EA); BZLF1 recombinant protein
Host
E. coli
Form/Format
Dry powder (lyophilized)
Applicable Applications for BZLF1 recombinant protein
ELISA, Western Blot (WB)
Application Notes
Suggested Titer by ELISA: Up to 1:1,183, which corresponds to 0.93 ug/ml of protein concentration in plates for IgG detection.
Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recomended to remove possible protein aggregates by centrifuging the stock solution, voiding alterations in the immobilization of the biomolecule to the solid surface.
Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recomended to remove possible protein aggregates by centrifuging the stock solution, voiding alterations in the immobilization of the biomolecule to the solid surface.
Molecular Weight Note
Determined by SDS-PAGE, the protein band is at the molecular marker between molecular markers of 45,000 Da and 35,000 Da, while relative molecular mass calculated from amino acid sequence is 35,081.1 Da.
Batch Composition
Components: his-zebra
Composition: recombinant antigen with a his-tag in its N-terminus
Components: Storage buffer befor lyophilization
Composition: 20 mM phosphate buffer pH8, 1M NaCl and 4 M urea.
Composition: recombinant antigen with a his-tag in its N-terminus
Components: Storage buffer befor lyophilization
Composition: 20 mM phosphate buffer pH8, 1M NaCl and 4 M urea.
Quality Control
Protein Concentration Determined Espectrophotometrically:
DO280 = 0.722
A0.1% (=1 g/l) = 0.653
Concentration*: 1.29 mg/ml
*The measurement of the protein concentration has been performed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989.
DO280 = 0.722
A0.1% (=1 g/l) = 0.653
Concentration*: 1.29 mg/ml
*The measurement of the protein concentration has been performed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989.
Reconstitution
With approx. 0.79 ml of sterile double-distilled water for a final concentration of approx. 1.10 mg/ml. The protein will be at pH 8.
Total Quantity per Aliquot
1 mg
Observation
proteins should be maintained frozen at high concentrations. The dilution to he performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Preparation and Storage
Protein is shipped on blue ice. Upon arrival, it should be stored at 4°C or -20°C. Once reconstituted, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.
ELISA
(Titration Curve by an ELISA Assay. The titer has been suggested in reference to an "in-house" ELISA kit performed at the lab over the first lot obtained. Each end user should carry out their own titration for their particular application. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgG sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera. )
ELISA
(Titration Curve by an ELISA Assay. The titer has been suggested in reference to an "in-house" ELISA kit performed at the lab over the first lot obtained. Each end user should carry out their own titration for their particular application. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgG sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera. )
SDS-PAGE
(SDS-PAGE analysis (15%) of 2 uL of recombinant ZEBRA. Purity is >98% as determined by gel electrophoresis. )
SDS-PAGE
(SDS-PAGE analysis (15%) of 2 uL of recombinant ZEBRA. Purity is >98% as determined by gel electrophoresis. )
Related Product Information for BZLF1 recombinant protein
Recombinant Antigen: Epstein-Barr virus zebra (Yamauchiet al., 1998)
The EBV gene BZLF1 has been expressed as a recombinant antigen zebra. It is produced from the complete ORF of the bZIP transcription factor.
The EBV gene BZLF1 has been expressed as a recombinant antigen zebra. It is produced from the complete ORF of the bZIP transcription factor.
Product Categories/Family for BZLF1 recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).
Similar Products
Product Notes
The BZLF1 (Catalog #AAA505029) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BZLF1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB). Suggested Titer by ELISA: Up to 1:1,183, which corresponds to 0.93 ug/ml of protein concentration in plates for IgG detection. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recomended to remove possible protein aggregates by centrifuging the stock solution, voiding alterations in the immobilization of the biomolecule to the solid surface. Researchers should empirically determine the suitability of the BZLF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BZLF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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