Testing Data
Eukaryotes Acid Phosphatase Cell Cytotoxicity Assay Kit
Acid Phosphatase Cell Cytotoxicity Assay Kit
Reactivity
Eukaryotes
Synonyms
Acid Phosphatase Cell Cytotoxicity; Acid Phosphatase Cell Cytotoxicity Assay Kit; Acid Phosphatase Cell Cytotoxicity assay kit
Reactivity
Eukaryotes
Samples
Cell culture: Adherent and suspensioncells
Detection Method
Absorbance: 405nm
Features & Benefits
Highly sensitive assay to measure Cell Cytotoxicity
In vitro cell proliferation cytotoxicity studies
High-throughput drug screening
The assay detects acid phosphatase (AP)activity as a direct correlation of cell cytotoxicity
In vitro cell proliferation cytotoxicity studies
High-throughput drug screening
The assay detects acid phosphatase (AP)activity as a direct correlation of cell cytotoxicity
Preparation and Storage
Store at 4 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Related Product Information for Acid Phosphatase Cell Cytotoxicity assay kit
The measurement of the lysosomal Acid Phosphatase (ACP) enzymatic activity has become one of the common methods used to detect and quantify cell viability or drug cytotoxicity due to ubiquitous of this enzymatic activity among all organisms. Under normal conditions, eukaryotic cells exhibit abundant cell-membrane associated ACP activity; however ACP activity is considerably lower when cells are exposed to cytotoxic agents. As a result, measurement of the ACP activity in cells is a reliable method to quantify cell growth in both adherent and non-adherent cells. Compared with other cell proliferatioin assays based on the reduction of tetrazolium salts, ACP cytotoxicity assay displays higher sensitivity and reproducibility. In this cell-based assay, intracellular ACP cleaves the colorless chromogenic substrate para-nitrophenyl phosphate producing a yellow colored product (p-nitrophenol) under alkaline conditions. The released chromophore can be measured at OD: 405 nm and is directly proportional to the viable cell number. ACP cytotoxicity assay kit is simple, accurate, reproducible and sensitive with a dynamic range between 103-105 cells. This kit offers a simple, yet excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening
Product Categories/Family for Acid Phosphatase Cell Cytotoxicity assay kit
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Product Notes
The Eukaryotes Acid Phosphatase Cell Cytotoxicity (Catalog #AAA849776) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849776 Assay Kit recognizes Eukaryotes Acid Phosphatase Cell Cytotoxicity. It is sometimes possible for the material contained within the vial of "Acid Phosphatase Cell Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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