Immunocytochemistry/Immunofluorescence (ICC/IF))
Mammalian Lysosomal Intracellular Assay Kit
Lysosomal Intracellular Activity Assay Kit
Reactivity
Mammalian
Synonyms
Lysosomal Intracellular; Lysosomal Intracellular Activity Assay Kit; Lysosomal Intracellular assay kit
Reactivity
Mammalian
Samples
Suspension or adherent cell cultures
Detection Method
Flow Cytometry: FL1
Microscopy Ex: 488nm
Microscopy Ex: 488nm
Features & Benefits
Measurement of lysosomal intracellular activity.
Elucidation of the mechanisms of endocytic pathway in living cells.
Screening compounds with anti-lysosomal intracellular activity
Measurement of lysosomal intracellular activity
Elucidation of the mechanisms of endocytic pathway in living cells.
Screening compounds with anti-lysosomal intracellular activity
Measurement of lysosomal intracellular activity
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Testing Data
Testing Data
Related Product Information for Lysosomal Intracellular assay kit
Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes that are utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. Although the intracellular activity of lysosome is difficult to measure in living cells, has developed a proprietary Lysosome-Specific Self-Quenched Substrate which has low background fluorescence, high signal to background ratio and is pH insensitive. The substrate, acting as endocytic cargo, can be taken up by cells and degraded within an endo-lysosomal vesicle. The fluorescent signal is recovered from the Self-Quenched Substrate. The fluorescence signal, generated by degradation, is proportional to the intracellular lysosomal activity and can be measured using a fluorescence microscopy and/or flow cytometry. Lysosomal Intracellular Activity Assay Kit (Cell-Based) includes Cytochalasin D, a cell-permeable inhibitor of endocytosis that serves as an experimental control. This easy-to-use, non-radioactive kit allows imaging and accurate measurement of de-quenching substrate in cultured cells
Product Categories/Family for Lysosomal Intracellular assay kit
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Product Notes
The Mammalian Lysosomal Intracellular (Catalog #AAA849671) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849671 Assay Kit recognizes Mammalian Lysosomal Intracellular. It is sometimes possible for the material contained within the vial of "Lysosomal Intracellular, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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