Typical Testing Data/Standard Curve (for reference only)
Peroxidase Assay Kit | AT1G49570 assay kit
Peroxidase Activity Colorimetric / Fluorometric Assay Kit
Gene Names
AT1G49570; F14J22.19; F14J22_19
Synonyms
Peroxidase; Peroxidase Activity Colorimetric / Fluorometric Assay Kit; AT1G49570 assay kit
Sequence Length
350
Samples
Cell and tissue culture supernatant, urine, serum and plasma, as well as many other biological fluids
Features & Benefits
Simple procedure
Fast and convenient
The assay is simple, direct, highly sensitive and high-throughput adaptable
Fast and convenient
The assay is simple, direct, highly sensitive and high-throughput adaptable
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AT1G49570 assay kit
Product Information: Peroxidase Activity Assay Kit: High-Throughput Colorimetric & Fluorometric Assay for detecting Peroxidase Activity in cell culture supernatant, serum, plasma, urine, and other biological fluids. Simple, Direct, Highly Sensitive. Detection Limit: 0.1 nmol (colorimetrically) & 0.01 nmol (fluorometrically). 100 Assays.
Background: Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ --> ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. MyBioSource's Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em=535/587)
Species reactivity- Mammalian
Application- The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Background: Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ --> ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. MyBioSource's Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em=535/587)
Species reactivity- Mammalian
Application- The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Product Categories/Family for AT1G49570 assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,030 Da
NCBI Official Full Name
peroxidase
NCBI Official Symbol
AT1G49570
NCBI Official Synonym Symbols
F14J22.19; F14J22_19
NCBI Protein Information
peroxidase
UniProt Protein Name
Peroxidase 10
Protein Family
UniProt Gene Name
PER10
UniProt Synonym Gene Names
P10; Atperox P10
UniProt Entry Name
PER10_ARATH
Uniprot Description
Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Similar Products
Product Notes
The AT1G49570 per10 (Catalog #AAA841574) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Peroxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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