Testing Data
WST-NR-CV Combined Cytotoxicity Assay Kit
WST-NR-CV Combined Cytotoxicity Assay Kit
Reactivity
Mammalian, Eukaryotes
Synonyms
WST-NR-CV Combined Cytotoxicity; WST-NR-CV Combined Cytotoxicity Assay Kit; WST-NR-CV Combined Cytotoxicity assay kit
Reactivity
Mammalian, Eukaryotes
Samples
Cell culture: Adherent cellsand non-adherent cells
Detection Method
Absorbance: OD: WST/NR/CV: 450 /540/570nm
Features & Benefits
Sensitive, Non-radioactive Assay, measures cytotoxicity using 3 different methods
Simple procedures; take less than 4 hours
Convenient
Uses a water-soluble tetrazolium salt WST reagent
Neutral Red and Crystal Violet.
Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Simple procedures; take less than 4 hours
Convenient
Uses a water-soluble tetrazolium salt WST reagent
Neutral Red and Crystal Violet.
Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for WST-NR-CV Combined Cytotoxicity assay kit
Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.
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Product Notes
The Mammalian, Eukaryotes WST-NR-CV Combined Cytotoxicity (Catalog #AAA849727) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849727 Assay Kit recognizes Mammalian, Eukaryotes WST-NR-CV Combined Cytotoxicity. It is sometimes possible for the material contained within the vial of "WST-NR-CV Combined Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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