Typical Testing Data/Standard Curve (for reference only)
InSitu HDAC Assay Kit
InSitu HDAC Activity Fluorometric Assay Kit
Synonyms
InSitu HDAC; InSitu HDAC Activity Fluorometric Assay Kit; InSitu HDAC assay kit
Samples
Cultured adherent or suspension cells
Features & Benefits
Simple procedure; just requires two easy steps
Direct, Fast, Fluorescence based
Kit contains all necessary reagents for measuring InSitu HDAC activity in adherent or suspension cells
Direct, Fast, Fluorescence based
Kit contains all necessary reagents for measuring InSitu HDAC activity in adherent or suspension cells
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for InSitu HDAC assay kit
Product Information: InSitu HDAC Activity Assay Kit: Measure InSitu HDAC Activity in adherent or suspension cells in Two Easy Steps. Fast, Direct, Fluorescence based. 100 assays.
Background: Histone acetylases (HAT's) and Histone deacetylases (HDAC's) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC's are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers. MyBioSource's InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. First, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.
Summary: Detection method- Fluorescence (Ex/Em = 368/442 nm)
Application: - Measurement of HDAC activity in cells in 96-well format - Screening HDAC inhibitors or activators - Studying growth factors or other regulators that influence HDAC activity
Background: Histone acetylases (HAT's) and Histone deacetylases (HDAC's) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC's are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers. MyBioSource's InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. First, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.
Summary: Detection method- Fluorescence (Ex/Em = 368/442 nm)
Application: - Measurement of HDAC activity in cells in 96-well format - Screening HDAC inhibitors or activators - Studying growth factors or other regulators that influence HDAC activity
Product Categories/Family for InSitu HDAC assay kit
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Product Notes
The InSitu HDAC (Catalog #AAA841506) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "InSitu HDAC, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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