Testing Data
Cell Cytotoxicity II Assay Kit
Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)
Reactivity
Mammalian, Eukaryotes
Synonyms
Cell Cytotoxicity II; Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet); Cell Cytotoxicity II assay kit
Reactivity
Mammalian, Eukaryotes
Samples
Cell culture: Adherent cellsand non-adherent cells
Detection Method
Absorbance: OD: WST/NR/CV: 450 /565/570nm
Features & Benefits
Sensitive, Non-radioactive Assay, measures cytotoxicity using 3 different methods
Simple procedures; take less than 4 hours
Convenient
Uses a water-soluble tetrazolium salt WST reagent, SRB and Crystal Violet.
Method are simple and do not require extensive washing/harvesting/or solubilization steps.
The entire assay can be performed directly in a 96-well plate.
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Simple procedures; take less than 4 hours
Convenient
Uses a water-soluble tetrazolium salt WST reagent, SRB and Crystal Violet.
Method are simple and do not require extensive washing/harvesting/or solubilization steps.
The entire assay can be performed directly in a 96-well plate.
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Cell Cytotoxicity II assay kit
Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), sulforhodamine B (SRB) or crystal violet (CV) are widely used in cultured cellsin order to assess the cytotoxicity of potential drug candidates as these methods are fast, reproducible, and do no require using living mammals during early drug discovery stages. However, the most critical problem of using single parameter assays is the determination of their IC50 data offers a limited perspective and can lead to erroneous assumptions or erroneous, unsucessful results when the selected candidates are used in more complex models (false positives). On the other hand, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. Thus, the analysis of candidates as potential cytotoxic compounds is usually more comprehensive when two or more cytotoxicity assays are performed. Additionally, SRB, WST and CV cyototoxic assays offer relatively inexpensive, accurate, yet reliable results in a timely manner. WST-SRB-Crystal Violet Combined Cytotoxicity Assay Kit allow researchers to test the cytotoxicity effect of their candidate compounds in different biological processes: cell proliferation (WST), protein synthesis (SRB) and/or total DNA synthesis (CV). The assays are compatible to each other and same set of cultured cells can be tested using these assays. The kit is simple, fast, high-throughput compatible and provides an efficient research tool for the quantitative measurement of cell cytotoxicity in terms of viability, protein and DNA synthesis
Product Categories/Family for Cell Cytotoxicity II assay kit
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Product Notes
The Mammalian, Eukaryotes Cell Cytotoxicity II (Catalog #AAA849562) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849562 Assay Kit recognizes Mammalian, Eukaryotes Cell Cytotoxicity II. It is sometimes possible for the material contained within the vial of "Cell Cytotoxicity II, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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