Sheep Forkhead Box Protein C1 ELISA Kit | FOXC1 elisa kit

Sheep Forkhead Box Protein C1 ELISA Kit

Cat. Num. AAA752867

• Gene Names: FOXC1; ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• Reactivity: Sheep
• Synonyms: Forkhead Box Protein C1; Sheep Forkhead Box Protein C1 ELISA Kit; FOXC1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This FOXC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FOXC1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This FOXC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FOXC1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXC1 elisa kit

Principle of the Assay: FOXC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOXC1 antibody and an FOXC1-HRP conjugate. The assay sample and buffer are incubated together with FOXC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOXC1 concentration since FOXC1 from samples and FOXC1-HRP conjugate compete for the anti-FOXC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOXC1 from the sample, fewer sites are left to bind FOXC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOXC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FOXC1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119395716
• NCBI GeneID: 2296
• NCBI Accession #: NP_001444.2
• NCBI GenBank Nucleotide #: NM_001453.2
• Molecular Weight: 56,789 Da
• NCBI Official Full Name: forkhead box protein C1
• NCBI Official Synonym Full Names: forkhead box C1
• NCBI Official Symbol: FOXC1
• NCBI Official Synonym Symbols: ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• NCBI Protein Information: forkhead box protein C1; myeloid factor-delta; forkhead box C1 protein; forkhead-related activator 3; forkhead-related protein FKHL7; forkhead, drosophila, homolog-like 7; forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7
• UniProt Protein Name: Forkhead box protein C1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC1
• UniProt Synonym Gene Names: FKHL7; FREAC3; FREAC-3
• UniProt Entry Name: FOXC1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: Transcription factor; Cell cycle regulation; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; DNA bending activity; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; glycosaminoglycan metabolic process; neural crest cell development; paraxial mesoderm formation; heart development; positive regulation of transcription, DNA-dependent; cardiac muscle cell proliferation; ovarian follicle development; regulation of transcription, DNA-dependent; ureteric bud development; germ cell migration; ventricular cardiac muscle morphogenesis; negative regulation of mitotic cell cycle; lacrimal gland development; regulation of blood vessel size; skeletal development; mesenchymal cell development; somitogenesis; ossification; Notch signaling pathway; cell migration; camera-type eye development; in utero embryonic development; regulation of organ growth; odontogenesis of dentine-containing teeth; cell proliferation; eye development; artery morphogenesis; embryonic heart tube development; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; brain development; lymph vessel development; vascular endothelial growth factor receptor signaling pathway

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1