BRPF1 recombinant protein

Recombinant BRPF1 (1077-1205), GST-Tag

Cat. Num. AAA388195

• Gene Names: BRPF1; BR140
• Purity: The recombinant protein is >92% pure by SDS-PAGE.
• Synonyms: BRPF1; Recombinant BRPF1 (1077-1205); GST-Tag; BR140; Peregrin; BRPF1 recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >92% pure by SDS-PAGE.
• Form/Format: Recombinant BRPF1 (1077-1205), GST-Tag protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

• Protein Species: Human
• Tag: GST-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant BRPF1 (1077-1205), GST-Tag protein gel. BRPF1 (1077-1205), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for BRPF1 recombinant protein

Short Description: The peptide corresponding to amino acids 1077-1205 that contains the PWWP domain sequences of BRPF1 (accession number NP_001003694.1) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.8 kDa. The recombinant protein is >92% pure by SDS-PAGE.

Background: Bromodomain and PHD finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2. BRPF1 possesses a PWWP (Pro-Trp-Trp-Pro) domain that functions in the association of PWWP domain-containing proteins with chromatin. PWWP domains have been shown to bind DNA and also specifically recognized methylated lysine residues of histones. In doing so they are suspected to be involved in the mediation of the cross-talk between DNA and histone epigenetic marks. Mutations in PWWP domains have been shown to be associated with various human diseases, including cancer.

Product Categories/Family for BRPF1 recombinant protein

Recombinant Proteins; Histone Acetyltransferase (HAT)

NCBI and Uniprot Product Information

• NCBI GI #: 51173719
• NCBI GeneID: 7862
• NCBI Accession #: NP_001003694.1
• NCBI GenBank Nucleotide #: NM_001003694
• Molecular Weight: 127,363 Da
• NCBI Official Full Name: Homo sapiens bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: bromodomain and PHD finger containing, 1
• NCBI Official Symbol: BRPF1
• NCBI Official Synonym Symbols: BR140
• NCBI Protein Information: peregrin; bromodomain and PHD finger-containing protein 1; bromodomain-containing protein, 140kD
• Protein Family: Peregrin

NCBI Description

The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]