beta-Glucosyltransferase Recombinant Protein | b-gt recombinant protein

Recombinant beta-Glucosyltransferase protein

Cat. Num. AAA389298

• Synonyms: beta-Glucosyltransferase; Recombinant beta-Glucosyltransferase protein; b-GT; beta-GT; b-gt recombinant protein

• Host: E Coli
• Form/Format: Recombinant beta-Glucosyltransferase protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.

• Protein Species: Escherichia virus T4
• Tag: His-Tag
• Protein Details: Beta-Glucosyltransferase was expressed in E Coli cells as the full length protein (accession number NP_049658.1) with an N-terminal 6xHis-tag.
• Notes: Recombinant beta-Glucosyltransferase protein is useful for the study of glucosyltransferase assays in vitro.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant beta-Glucosyltransferase protein 10% SDS-PAGE gel with Coomassie Blue staining MW: 42.8 kDa Purity: ?90%)

Related Product Information for b-gt recombinant protein

Short Description: Beta-Glucosyltransferase, also called BGT, is a DNA-modifying enzyme. It is encoded by a number of bacteriophages belonging to the T-even group. It catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. BGT shows little DNA sequence specificity but modify only 5-HMC bases. The glucosylation protects the infecting viral DNA from host nucleases. In addition to its protective role, the glucosylation has also been implicated in the control of phage-specific gene expression by influencing transcription in vivo and in vitro.

Background: Beta-Glucosyltransferase, also called as b-Glucosyltransferase or BGT, is a DNA-modifying enzyme. It is encoded by a number of bacteriophages belonging to the T-even group. It catalyses the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. BGT shows little DNA sequence specificity but modify only 5-HMC bases. The glucosylation protects the infecting viral DNA from host nucleases. In addition to its protective role, the glucosylation has also been implicated in the control of phage-specific gene expression by influencing transcription in vivo and in vitro. The reactions is: UDP-Glc + 5-HMC-DNA --> b-glucosyl- HMC-DNA + UDP.

Product Categories/Family for b-gt recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 9632714
• NCBI GeneID: 1258765
• NCBI Accession #: NP_049658.1
• NCBI GenBank Nucleotide #: NC_000866.4
• UniProt Accession #: P04547; Q38417
• Molecular Weight: 40,666 Da
• NCBI Official Full Name: b-gt beta glucosyl transferase
• NCBI Official Symbol: b-gt
• NCBI Protein Information: adds a glucose in beta linkage to hydroxymethylcytosine residues in phage DNA
• UniProt Protein Name: DNA beta-glucosyltransferase
• UniProt Gene Name: bgt
• UniProt Synonym Gene Names: BGT; Beta-GT

Uniprot Description

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC) (PubMed:22229759). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45 (PubMed:22229759). The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system (PubMed:22229759). The glc-HMC modification also protects against the host CRISPR-Cas9 defense system (PubMed:26081634).