ALKBH8 recombinant protein

Recombinant ALKBH8 protein

Cat. Num. AAA389250

• Gene Names: ALKBH8; ABH8; TRM9; TRMT9; TRMT9A
• Synonyms: ALKBH8; Recombinant ALKBH8 protein; AlkB Homolog 8; TRNA Methyltransferase; S-Adenosyl-L-Methionine-Dependent TRNA; ALKBH8 recombinant protein

• Host: E Coli
• Form/Format: Recombinant ALKBH8 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant ALKBH8 protein gel 10% SDS-PAGE Coomassie staining MW: 77.9 kDa Purity: >48%)

Related Product Information for ALKBH8 recombinant protein

Short Description: Full-length ALKBH8 protein (accession number NP_001287939.1) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of ALKBH8 is 77.9 kDa.

Background: ALKBH8 (AlkB Homolog 8), also called as ABH8 or TRMT9A, is a S-Adenosyl-L-Methionine-dependent tRNA methyltransferase. It catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. It was reported to have a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). ALKBH8 can bind tRNA and catalyze the iron and alphaketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine. It is also required for normal survival after DNA damage. ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis.

Product Categories/Family for ALKBH8 recombinant protein

Recombinant Proteins; DNA Damage; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 666335573
• NCBI GeneID: 91801
• NCBI Accession #: NP_001287939.1
• NCBI GenBank Nucleotide #: NM_001301010.1
• UniProt Accession #: Q96BT7; Q8N989; B1Q2M0; B4DEF6
• Molecular Weight: 75,558 Da
• NCBI Official Full Name: alkylated DNA repair protein alkB homolog 8 isoform 1
• NCBI Official Synonym Full Names: alkB homolog 8, tRNA methyltransferase
• NCBI Official Symbol: ALKBH8
• NCBI Official Synonym Symbols: ABH8; TRM9; TRMT9; TRMT9A
• NCBI Protein Information: alkylated DNA repair protein alkB homolog 8
• UniProt Protein Name: Alkylated DNA repair protein alkB homolog 8
• Protein Family: Alkylated DNA repair protein
• UniProt Gene Name: ALKBH8
• UniProt Synonym Gene Names: ABH8
• UniProt Entry Name: ALKB8_HUMAN

Uniprot Description

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.229; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding

Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation