NSUN4 recombinant protein
Recombinant NSUN4 (SHTAP) protein
Synonyms
NSUN4; Recombinant NSUN4 (SHTAP) protein; NOP2/Sun Domain Family; Member 4; NSUN4 recombinant protein
Host
Baculovirus
Form/Format
Recombinant NSUN4 (SHTAP) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant NSUN4 (SHTAP) protein 10% SDS-PAGE Coomassie staining MW: 44.4 kDa Purity: >80%)
Related Product Information for NSUN4 recombinant protein
Short Description: Recombinant NSUN4 (SHTAP) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_950245.2) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 44.4 kDa.
Background: NSUN4 (NOP2/Sun Domain Family, Member 4), also called as SHTAP, is a member of NSUN family. It is involved in mitochondrial ribosome assembly. NSUN4 is a 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). It is targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro, NSUN4 can methylate 16S rRNA of the LSU, which can be enhanced by MTERFD/MTERF4.
Background: NSUN4 (NOP2/Sun Domain Family, Member 4), also called as SHTAP, is a member of NSUN family. It is involved in mitochondrial ribosome assembly. NSUN4 is a 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). It is targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro, NSUN4 can methylate 16S rRNA of the LSU, which can be enhanced by MTERFD/MTERF4.
Product Categories/Family for NSUN4 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 44.4kDa
Protein Family
