SDS-Page
(Recombinant dCas9 protein, His/AM Tag 7.5% SDS-PAGE gel stained with Coomassie Blue MW: 166 kDa Purity: > 80% )
dCas9 recombinant protein
Recombinant dCas9 protein, His/AM Tag
Synonyms
dCas9; Recombinant dCas9 protein; His/AM Tag; Clustered Regularly Interspersed Short Palindromic Repeasts; CRISPR associated protein 9; dCas9 recombinant protein
Host
E Coli
Form/Format
Recombinant dCas9 protein, His/AM tag is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
Protein Species
S. pyogenes
Tag
His-Tag
Notes
This protein is useful for the study of DNA binding, transcriptional activation/repression as well as other genome engineering.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant dCas9 protein, His/AM Tag 7.5% SDS-PAGE gel stained with Coomassie Blue MW: 166 kDa Purity: > 80% )
SDS-Page
(Recombinant dCas9 protein, His/AM Tag 7.5% SDS-PAGE gel stained with Coomassie Blue MW: 166 kDa Purity: > 80% )
Related Product Information for dCas9 recombinant protein
Short Description: Recombinant dCas9 protein, His/AM tag was expressed in E Coli as the full-length protein (accession number WP_010922251.1) with two point mutations (Glutamic acid to Alanine at amino acid 10 and Histidine to Alanine at amino acid 840). The protein has an N-terminal 6xHis-tag and a C-terminal nuclear localization sequence then two copies of AM tag. The molecular weight of the protein is 166 kDa. This protein is useful for the study of DNA binding, transcriptional activation/repression as well as other genome engineering.
Background: dCas9 (deficient Cas9) protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). Cas9 is an RNA-guided DNA endonuclease associated with adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 can unwind foreign DNA and checking for sites complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription. While native Cas9 requires a guide RNA composed of two disparate RNAs that associate to make the guide-the CRISPR RNA (crRNA), and the trans-activating RNA (tracrRNA), dCas9 targeting has been simplified through the engineering of a chimeric single guide RNA (chiRNA).
Background: dCas9 (deficient Cas9) protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). Cas9 is an RNA-guided DNA endonuclease associated with adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 can unwind foreign DNA and checking for sites complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription. While native Cas9 requires a guide RNA composed of two disparate RNAs that associate to make the guide-the CRISPR RNA (crRNA), and the trans-activating RNA (tracrRNA), dCas9 targeting has been simplified through the engineering of a chimeric single guide RNA (chiRNA).
Product Categories/Family for dCas9 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 166kDa
Similar Products
Product Notes
The dCas9 (Catalog #AAA389127) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "dCas9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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