CENPB recombinant protein

Recombinant CENPB protein

Cat. Num. AAA389253

• Synonyms: CENPB; Recombinant CENPB protein; Centromere Protein B; Major Centromere Autoantigen B; CENPB recombinant protein

• Host: E Coli
• Form/Format: Recombinant CENPB protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant CENPB protein gel 10% SDS-PAGE Coomassie staining MW: 68.7 kDa Purity: >85%)

Related Product Information for CENPB recombinant protein

Short Description: Recombinant human CENPB protein was expressed in E Coli as the full-length protein (accession number NP_001801) with an N-terminal 6xHis-tag. The molecular weight of the protein is 68.7 kDa.

Background: CENPB (Centromere Protein B), also called as CENP-B, is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. CENPB can interact with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. It may organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. CENPB is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Product Categories/Family for CENPB recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21735415
• NCBI GeneID: 1059
• NCBI Accession #: NP_001801
• NCBI GenBank Nucleotide #: NM_001810
• UniProt Accession #: P07199
• Molecular Weight: 65kDa
• NCBI Official Full Name: major centromere autoantigen B
• NCBI Official Synonym Full Names: centromere protein B
• NCBI Official Symbol: CENPB
• NCBI Protein Information: major centromere autoantigen B
• UniProt Protein Name: Major centromere autoantigen B
• Protein Family: CENPB DNA-binding domain-containing protein
• UniProt Gene Name: CENPB
• UniProt Synonym Gene Names: CENP-B
• UniProt Entry Name: CENPB_HUMAN

NCBI Description

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: chromosome; nucleus; chromosome, pericentric region

Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding

Biological Process: regulation of transcription, DNA-dependent