SDS-Page
(Recombinant Ketohexokinase protein gel 10% SDS-PAGE with Coomassie blue staining MW: 33.8 kDa Purity: >92%)
Ketohexokinase Recombinant Protein | KHK recombinant protein
Recombinant Ketohexokinase protein
Synonyms
Ketohexokinase; Recombinant Ketohexokinase protein; KHK; fructokinase; KHK recombinant protein
Host
E Coli
Form/Format
Recombinant Ketohexokinase protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
Protein Species
Human
Tag
His-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant Ketohexokinase protein gel 10% SDS-PAGE with Coomassie blue staining MW: 33.8 kDa Purity: >92%)
SDS-Page
(Recombinant Ketohexokinase protein gel 10% SDS-PAGE with Coomassie blue staining MW: 33.8 kDa Purity: >92%)
Related Product Information for KHK recombinant protein
Short Description: Recombinant Ketohexokinase protein was expressed in E Coli as the full-length protein (accession number NP_000212.1) with a C-terminal 6xHis-tag. The molecular weight of the protein is 33.8 kDa.
Background: Ketohexokinase, also called as Fructokinase or KHK, can catalyze the phosphorylation of the ketose sugar fructose to fructose-1-phosphate. A number of other furanose sugars can also act as KHK substrates. KHK has two isoforms, KHK-A and KHK-C. KHK-C has a 10-fold higher affinity for fructose (Km = 0.8 mM) and is mainly responsible for its metabolism..KHK-C is expressed primarily in liver while KHK-A is expressed at low levels in a wide range of tissues, and its physiological substrate remains unknown. Liver is the primary organ that metabolizes most of the ingested fructose, the small intestine strongly expresses all fructose-metabolizing enzymes and is responsible for the catabolism of 10-30% of ingested fructose. Fructolysis is initiated by KHK which converts fructose and ATP into fructose 1-phosphate and ADP, respectively. Aldolase-B cleaves fructose 1-phosphate into three-carbon intermediates, dihydroxyacetone phosphate and glyceraldehyde, and the latter is then converted by triokinase into glyceraldehyde 3-phosphate, which then joins the glycolysis pathway. Fructose catabolism initiated by KHK therefore bypasses important glycolytic regulatory steps in glycolysisthatgeneratefructose1,6- bisphosphatethroughtheaction oftheenergy-sensitiveenzymephosphofructokinase(PFK),resulting in greater lipogenesis than that resulting from glycolysis-regulated lipid production. In addition, fructose catabolism bypasses the glucose-6- phosphate and fructose-6-phosphate-derived pentose phosphate pathway (PPP), which produces ribose-5-phosphate (R5P) for de novo synthesis of nucleotides and nucleic acids. The KHK product fructose-1-phosphate relieves the binding of glucokinase to GCKR, increasing glucokinase availability and promoting its translocation from sequestered nuclear to cytoplasmic localization. GCKR regulates glucokinase primarily in the liver but may play a similar role in the pancreatic islet and hypothalamus, and it has been proposed to modulate glucose sensing.
Background: Ketohexokinase, also called as Fructokinase or KHK, can catalyze the phosphorylation of the ketose sugar fructose to fructose-1-phosphate. A number of other furanose sugars can also act as KHK substrates. KHK has two isoforms, KHK-A and KHK-C. KHK-C has a 10-fold higher affinity for fructose (Km = 0.8 mM) and is mainly responsible for its metabolism..KHK-C is expressed primarily in liver while KHK-A is expressed at low levels in a wide range of tissues, and its physiological substrate remains unknown. Liver is the primary organ that metabolizes most of the ingested fructose, the small intestine strongly expresses all fructose-metabolizing enzymes and is responsible for the catabolism of 10-30% of ingested fructose. Fructolysis is initiated by KHK which converts fructose and ATP into fructose 1-phosphate and ADP, respectively. Aldolase-B cleaves fructose 1-phosphate into three-carbon intermediates, dihydroxyacetone phosphate and glyceraldehyde, and the latter is then converted by triokinase into glyceraldehyde 3-phosphate, which then joins the glycolysis pathway. Fructose catabolism initiated by KHK therefore bypasses important glycolytic regulatory steps in glycolysisthatgeneratefructose1,6- bisphosphatethroughtheaction oftheenergy-sensitiveenzymephosphofructokinase(PFK),resulting in greater lipogenesis than that resulting from glycolysis-regulated lipid production. In addition, fructose catabolism bypasses the glucose-6- phosphate and fructose-6-phosphate-derived pentose phosphate pathway (PPP), which produces ribose-5-phosphate (R5P) for de novo synthesis of nucleotides and nucleic acids. The KHK product fructose-1-phosphate relieves the binding of glucokinase to GCKR, increasing glucokinase availability and promoting its translocation from sequestered nuclear to cytoplasmic localization. GCKR regulates glucokinase primarily in the liver but may play a similar role in the pancreatic islet and hypothalamus, and it has been proposed to modulate glucose sensing.
Product Categories/Family for KHK recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ketohexokinase isoform a
NCBI Official Synonym Full Names
ketohexokinase (fructokinase)
NCBI Official Symbol
KHK
NCBI Protein Information
ketohexokinase; hepatic fructokinase
UniProt Protein Name
Ketohexokinase
Protein Family
UniProt Gene Name
KHK
UniProt Entry Name
KHK_HUMAN
NCBI Description
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
KHK iso2: Defects in KHK are the cause of fructosuria (FRUCT). Benign defect of intermediary metabolism. Belongs to the carbohydrate kinase PfkB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.3; Carbohydrate Metabolism - fructose and mannose; Transferase
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: ketohexokinase activity; ATP binding
Biological Process: carbohydrate phosphorylation; carbohydrate metabolic process; pathogenesis; fructose catabolic process
Disease: Fructosuria, Essential
Research Articles on KHK
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Product Notes
The KHK khk (Catalog #AAA389345) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ketohexokinase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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