SDS-Page
(Recombinant SARS-CoV-2 NSP16 protein gel 12.5% SDS-PAGE with Coomassie blue staining MW: 34.5 kDa Purity: >88%)
COVID 19 NSP16 Coronavirus Recombinant Protein | COVID-19 recombinant protein
Recombinant SARS-CoV-2 NSP16 protein
Synonyms
COVID 19 NSP16 Coronavirus; Recombinant SARS-CoV-2 NSP16 protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP16; COVID-19 recombinant protein
Host
E Coli
Form/Format
Recombinant SARS-CoV-2 NSP16 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
Protein Species
SARS-CoV-2
Notes
Recombinant SARS-CoV-2 NSP16 protein is suitable for use in the study of SARS-CoV-2. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SARS-CoV-2 NSP16 protein gel 12.5% SDS-PAGE with Coomassie blue staining MW: 34.5 kDa Purity: >88%)
SDS-Page
(Recombinant SARS-CoV-2 NSP16 protein gel 12.5% SDS-PAGE with Coomassie blue staining MW: 34.5 kDa Purity: >88%)
Related Product Information for COVID-19 recombinant protein
Short Description: Recombinant SARS-CoV-2 NSP16 protein was expressed in E Coli as the full-length protein (accession number YP_009725311.1) with a C-terminal 6xHis-tag. The predicted molecular weight of the protein is 34.5 kDa. Recombinant SARS-CoV-2 NSP16 protein is suitable for use in the study of SARS-CoV-2.
Background: SARS-CoV-2 NSP16 (Nonstructural Protein 16), also called as ribose 2'-O-methyltransferase, is one of the nonstructural proteins encoded by SARS-CoV-2 orf1ab. The polyproteins of CoVs are cleaved by virus-encoded cysteine proteinases comprise papain- and chymotrypsin-like proteases into 16 nonstructural proteins including the expression of NSP1 to NSP11 by orf1a and encoding NSP12 to NSP16 by orf1b. According to BLAST analysis, the sequence identity of ORF1ab protein between SARS-CoV-2 and SARS-CoV is more than 90% with the query cover of about 100%, while the sequence identity of NSP16 between these two viruses is about 93.24%. The 5'-cap structure is a distinct feature of eukaryotic mRNAs, and eukaryotic viruses generally modify the 5'-end of viral RNAs to mimic cellular mRNA structure, which is important for RNA stability, protein translation and viral immune escape. SARS coronavirus (SARS-CoV) encodes two S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTase) which sequentially methylate the RNA cap at guanosine-N7 and ribose 2'-O positions, catalyzed by NSP14 N7-MTase and NSP16 2'-O-MTase, respectively. A unique feature for SARS-CoV is that NSP16 requires non-structural protein NSP10 as a stimulatory factor to execute its MTase activity. Similarly, SARS-CoV-2 NSP16 is estimated to be a methyltransferase to methylate the RNA cap when complexed with NSP10.
Background: SARS-CoV-2 NSP16 (Nonstructural Protein 16), also called as ribose 2'-O-methyltransferase, is one of the nonstructural proteins encoded by SARS-CoV-2 orf1ab. The polyproteins of CoVs are cleaved by virus-encoded cysteine proteinases comprise papain- and chymotrypsin-like proteases into 16 nonstructural proteins including the expression of NSP1 to NSP11 by orf1a and encoding NSP12 to NSP16 by orf1b. According to BLAST analysis, the sequence identity of ORF1ab protein between SARS-CoV-2 and SARS-CoV is more than 90% with the query cover of about 100%, while the sequence identity of NSP16 between these two viruses is about 93.24%. The 5'-cap structure is a distinct feature of eukaryotic mRNAs, and eukaryotic viruses generally modify the 5'-end of viral RNAs to mimic cellular mRNA structure, which is important for RNA stability, protein translation and viral immune escape. SARS coronavirus (SARS-CoV) encodes two S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTase) which sequentially methylate the RNA cap at guanosine-N7 and ribose 2'-O positions, catalyzed by NSP14 N7-MTase and NSP16 2'-O-MTase, respectively. A unique feature for SARS-CoV is that NSP16 requires non-structural protein NSP10 as a stimulatory factor to execute its MTase activity. Similarly, SARS-CoV-2 NSP16 is estimated to be a methyltransferase to methylate the RNA cap when complexed with NSP10.
Product Categories/Family for COVID-19 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 34.5kDa
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Product Notes
The COVID-19 (Catalog #AAA389363) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 NSP16 Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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