SDS-Page
(Recombinant NSUN3 protein 10% SDS-PAGE Coomassie staining MW: 39.5 kDa Purity: >80%)
NSUN3 recombinant protein
Recombinant NSUN3 protein
Gene Names
NSUN3; MST077; MSTP077
Synonyms
NSUN3; Recombinant NSUN3 protein; NOP2/Sun RNA Methyltransferase Family Member 3; NSUN3 recombinant protein
Host
Baculovirus
Form/Format
Recombinant NSUN3 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant NSUN3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant NSUN3 protein 10% SDS-PAGE Coomassie staining MW: 39.5 kDa Purity: >80%)
SDS-Page
(Recombinant NSUN3 protein 10% SDS-PAGE Coomassie staining MW: 39.5 kDa Purity: >80%)
Related Product Information for NSUN3 recombinant protein
Short Description: Recombinant NSUN3 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_071355.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 39.5 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: NSUN3 (NOP2/Sun RNA Methyltransferase Family Member 3), is a member of NSUN family. Gene Ontology (GO) annotations related to this gene include RNA binding and methyltransferase activity. NSUN3 is said to be a mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.
Background: NSUN3 (NOP2/Sun RNA Methyltransferase Family Member 3), is a member of NSUN family. Gene Ontology (GO) annotations related to this gene include RNA binding and methyltransferase activity. NSUN3 is said to be a mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.
Product Categories/Family for NSUN3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,244 Da
NCBI Official Full Name
tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
NCBI Official Synonym Full Names
NOP2/Sun RNA methyltransferase family member 3
NCBI Official Symbol
NSUN3
NCBI Official Synonym Symbols
MST077; MSTP077
NCBI Protein Information
tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
UniProt Protein Name
tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
Protein Family
UniProt Gene Name
NSUN3
Uniprot Description
NSUN3: May have S-adenosyl-L-methionine-dependent methyl- transferase activity. Belongs to the methyltransferase superfamily. RsmB/NOP family.
Protein type: EC 2.1.1.-; Methyltransferase
Chromosomal Location of Human Ortholog: 3q11.2
Cellular Component: mitochondrial large ribosomal subunit; mitochondrial matrix; mitochondrion
Molecular Function: tRNA (cytosine-5-)-methyltransferase activity; tRNA binding
Biological Process: rRNA methylation
Research Articles on NSUN3
Similar Products
Product Notes
The NSUN3 nsun3 (Catalog #AAA389238) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NSUN3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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