SDS-Page
(Recombinant METTL1 / WDR4 Complex 10% SDS-PAGE with Coomassie blue staining MW of WDR4: 45.5 kDa MW of METTL1: 32.7 kDa Purity: >90%)
METTL1/WDR4 recombinant protein
Recombinant METTL1/WDR4 complex
Synonyms
METTL1/WDR4; Recombinant METTL1/WDR4 complex; METTL1/WDR4 recombinant protein
Host
Baculovirus
Form/Format
Recombinant METTL1/WDR4 Complex is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant METTL1 / WDR4 Complex 10% SDS-PAGE with Coomassie blue staining MW of WDR4: 45.5 kDa MW of METTL1: 32.7 kDa Purity: >90%)
SDS-Page
(Recombinant METTL1 / WDR4 Complex 10% SDS-PAGE with Coomassie blue staining MW of WDR4: 45.5 kDa MW of METTL1: 32.7 kDa Purity: >90%)
Related Product Information for METTL1/WDR4 recombinant protein
Short Description: Recombinant METTL1/WDR4 complex that includes full-length human WDR4 protein (accession number NP_061139.2) and full-length human METTL1 protein (accession number NP_005362.3) with an N-terminal DYKDDDDK-Tag was expressed in a baculovirus expression system. The molecular weights of WDR4 and METTL1 are 45.5 kDa and 32.7 kDa, respectively.
Background: WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his andtrp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. METTL1 (Methyltransferase-Like Protein 1), also known as TRM8, contains a conserved S-adenosylmethionine-binding motif and is inactivated by the protein kinase B (PKB) and ribosomal S6 kinase (RSK) under growth factor stimulation. WRD4 and METTL1 that form a complex are required for tRNA (guanine(46)-N(7))-methyltransferase activity. More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modification occurs at position 46 in the variable region and is a product of tRNA (m7G46) methyltransferase. The m7G46 modification forms a tertiary base pair with C13-G22, and stabilizes the tRNA structure.
Background: WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his andtrp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. METTL1 (Methyltransferase-Like Protein 1), also known as TRM8, contains a conserved S-adenosylmethionine-binding motif and is inactivated by the protein kinase B (PKB) and ribosomal S6 kinase (RSK) under growth factor stimulation. WRD4 and METTL1 that form a complex are required for tRNA (guanine(46)-N(7))-methyltransferase activity. More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modification occurs at position 46 in the variable region and is a product of tRNA (m7G46) methyltransferase. The m7G46 modification forms a tertiary base pair with C13-G22, and stabilizes the tRNA structure.
Product Categories/Family for METTL1/WDR4 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weights of WDR4 and METTL1 are 45.5 kDa and 32.7 kDa
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Product Notes
The METTL1/WDR4 (Catalog #AAA389255) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "METTL1/WDR4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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