Typical Testing Data/Standard Curve (for reference only)
Thrombin Assay Kit
Thrombin Activity Fluorometric Assay Kit
Synonyms
Thrombin; Thrombin Activity Fluorometric Assay Kit; Thrombin assay kit
Sequence Length
36
Application Notes
Determine activity of pure Thrombin
Detect the activity of Thrombin in plasma
Detect the activity of Thrombin in plasma
Samples
Plasma
Detection Method
Fluorescence (Ex/Em = 350/450 nm)
Features & Benefits
Simple & convenient protocol.
Can detect thrombin activity as low as.
Kit contains all necessary reagents for measuring thrombin activity.
Can detect thrombin activity as low as.
Kit contains all necessary reagents for measuring thrombin activity.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Thrombin assay kit
Product Information: Thrombin Activity Fluorometric Assay Kit: simple and rapid assay to measure thrombin (Factor Iia) activity. 100 Assays.
Background: Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin. Thrombin is also a potent vasoconstrictor and mitogen implicated as a major factor in vasospasm following subarachnoid hemorrhage. Ruptured cerebral aneurysm blood clots around a cerebral artery releases thrombin, which in turn induces acute and prolonged narrowing of the blood vessel, potentially resulting in cerebral ischemia and infarction (stroke). In addition, it is a pro-inflammatory enzyme that may influence the onset and progression of atherosclerosis. MyBioSource's Thrombin activity assay kit utilizes the ability of Thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence reader. This assay kit is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
Summary: Detection method- Fluorescence (Ex/Em = 350/450 nm)
Applications- Determine activity of pure Thrombin - Detect the activity of Thrombin in plasma
Background: Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin. Thrombin is also a potent vasoconstrictor and mitogen implicated as a major factor in vasospasm following subarachnoid hemorrhage. Ruptured cerebral aneurysm blood clots around a cerebral artery releases thrombin, which in turn induces acute and prolonged narrowing of the blood vessel, potentially resulting in cerebral ischemia and infarction (stroke). In addition, it is a pro-inflammatory enzyme that may influence the onset and progression of atherosclerosis. MyBioSource's Thrombin activity assay kit utilizes the ability of Thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence reader. This assay kit is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
Summary: Detection method- Fluorescence (Ex/Em = 350/450 nm)
Applications- Determine activity of pure Thrombin - Detect the activity of Thrombin in plasma
Product Categories/Family for Thrombin assay kit
NCBI and Uniprot Product Information
Uniprot Description
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing ().
Similar Products
Product Notes
The Thrombin (Catalog #AAA841755) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Determine activity of pure Thrombin Detect the activity of Thrombin in plasma. Researchers should empirically determine the suitability of the Thrombin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombin, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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