Typical Testing Data/Standard Curve (for reference only)
Factor VIIIa Assay Kit
Factor VIIIa Activity Assay Kit (Fluorometric)
Synonyms
Factor VIIIa; Factor VIIIa Activity Assay Kit (Fluorometric); Factor VIIIa assay kit
Application Notes
Determine activity of pure FVIIIa
Detect activity of FVIIIa in plasma, and serum
Detect activity of FVIIIa in plasma, and serum
Samples
Purified enzyme
Serum, plasma
Serum, plasma
Detection Method
Fluorescence (Ex/Em = 360/450 nm)
Features & Benefits
Convenient, and rapid
The assay can detect activity as low as 1 ng of FVIIIa in a variety of samples.
The assay can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Factor VIIIa assay kit
Product Information: Factor VIIIa Activity Assay Kit (Fluorometric): Convenient assay to measure Factor VIIIa activity of plasma and serum samples or pure Factor VIIIa. Detection Limit: 1 ng of Factor VIIIa, 100 assays.
Background: The coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa). MyBioSource's Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 360/450 nm)
Applications: - Determine activity of pure FVIIIa - Detect activity of FVIIIa in plasma, and serum
Background: The coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa). MyBioSource's Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 360/450 nm)
Applications: - Determine activity of pure FVIIIa - Detect activity of FVIIIa in plasma, and serum
Product Categories/Family for Factor VIIIa assay kit
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Product Notes
The Factor VIIIa (Catalog #AAA841487) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Determine activity of pure FVIIIa Detect activity of FVIIIa in plasma, and serum. Researchers should empirically determine the suitability of the Factor VIIIa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor VIIIa, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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