Testing Data
Eukaryotes Hypoxanthine Phosphoribosyl Transferase Assay Kit
PicoProbe Hypoxanthine Phosphoribosyl Transferase Activity Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
Hypoxanthine Phosphoribosyl Transferase; PicoProbe Hypoxanthine Phosphoribosyl Transferase Activity Assay Kit (Fluorometric); Hypoxanthine Phosphoribosyl Transferase assay kit
Reactivity
Eukaryotes
Samples
Cell lysate (eg. Jurkat cell lysate)
Tissue lysate (eg. Liver tissue)
Recombinant enzyme
Purified protein
Tissue lysate (eg. Liver tissue)
Recombinant enzyme
Purified protein
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
Highly Sensitive Assay for measuring HPRT
Simple, rapid & convenient assay to measure HPRTActivity in purified enzyme
Includes Positive Control
Recombinant enzyme
Purified protein
Measurement of Hypoxanthine Phosphoribosyl Transferase activity in purified/crude enzyme/cell lysate/tissue lysate preparations
Simple, rapid & convenient assay to measure HPRTActivity in purified enzyme
Includes Positive Control
Recombinant enzyme
Purified protein
Measurement of Hypoxanthine Phosphoribosyl Transferase activity in purified/crude enzyme/cell lysate/tissue lysate preparations
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Hypoxanthine Phosphoribosyl Transferase assay kit
Hypoxanthine Phosphoribosyl Transferase or HPRT (EC 2.4.2.8) plays an important role in the generation of purine nucleotides through the purine salvage pathway and is present in prokaryotes and eukaryotes. The enzyme converts guanine to guanosine monophosphate and hypoxanthine to inosine monophosphate by transferring a phosphoribosyl group from phosphoribosyl pyrophosphate (PRPP) to the purine molecule, and releases an inorganic pyrophosphate molecule in the process. Mutations in HPRT lead to developmental disorders such as Lesch Nyhan Disease (LND), characterized by neurological and behavioral abnormalities. Its deficiency also leads to defective basal ganglia expression of the neurotransmitter dopamine and aberrant neuronal function. This causes dysregulation of multiple dopamine- related developmental functions and cellular signaling defects. HPRT Activity Assay Kit is a simple one-step plate based assay kit for the measurement of HPRT activity in biological samples. HPRT catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP). IMP then undergoes a series of enzymatic reactions to convert a non-fluorescent probe to a fluorescent product. The signal, which is proportional to the generated product can be read in kinetic mode at Ex/Em 535/587 nm. The assay can detect as low as 2 mU HPRT.
Product Categories/Family for Hypoxanthine Phosphoribosyl Transferase assay kit
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Product Notes
The Eukaryotes Hypoxanthine Phosphoribosyl Transferase (Catalog #AAA849695) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849695 Assay Kit recognizes Eukaryotes Hypoxanthine Phosphoribosyl Transferase. It is sometimes possible for the material contained within the vial of "Hypoxanthine Phosphoribosyl Transferase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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