Typical Testing Data/Standard Curve (for reference only)
Factor IXa Assay Kit
Factor IXa Activity Assay Kit (Fluorometric)
Synonyms
Factor IXa; Factor IXa Activity Assay Kit (Fluorometric); Factor IXa assay kit
Application Notes
Determine activity of purified Factor IXa
Detect the activity of Factor IXa in plasma and serum samples
Detect the activity of Factor IXa in plasma and serum samples
Samples
Purified Factor Ixa, Plasma, Serum
Detection Method
Fluorescence (Ex/Em = 360/450 nm
Features & Benefits
Rapid and convenient
The assay can detect Factor IXa activity as low as 10 pg.
The assay can detect Factor IXa activity as low as 10 pg.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Factor IXa assay kit
Product Information: Factor IXa Activity Fluorometric Assay Kit: Convenient assay to measure Factor IXa activity of purified Factor Ixa or in plasma or serum samples. Detection Limit: 10 pg, 100 assays.
Background: The coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). MyBioSource's Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 10 pg of FIXa in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 360/450 nm
Applications: - Determine activity of purified Factor IXa - Detect the activity of Factor IXa in plasma and serum samples
Background: The coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). MyBioSource's Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 10 pg of FIXa in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 360/450 nm
Applications: - Determine activity of purified Factor IXa - Detect the activity of Factor IXa in plasma and serum samples
Product Categories/Family for Factor IXa assay kit
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Product Notes
The Factor IXa (Catalog #AAA841526) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Determine activity of purified Factor IXa Detect the activity of Factor IXa in plasma and serum samples. Researchers should empirically determine the suitability of the Factor IXa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor IXa, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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