Typical Testing Data/Standard Curve (for reference only)
PicoProbe Aldehyde Dehydrogenase Assay Kit
PicoProbe Aldehyde Dehydrogenase Activity Fluorometric Assay Kit
Synonyms
PicoProbe Aldehyde Dehydrogenase; PicoProbe Aldehyde Dehydrogenase Activity Fluorometric Assay Kit; PicoProbe Aldehyde Dehydrogenase assay kit
Samples
Cell and tissue culture supernatants, cell lysates, plasma and serum, as well as many other biological fluids etc.
Features & Benefits
Simple procedure
Fast and convenient
Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Fast and convenient
Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe Aldehyde Dehydrogenase assay kit
Product Information: PicoProbe Aldehyde Dehydrogenase Activity Assay Kit: Fluorometric Assay for Measuring ALDH Activity in various Biological Samples such as Tissues, Cells etc. Rapid, Convenient & Highly Sensitive. Detection Limit: < 0.05 mU. 100 Assays.
Background: The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis. Therefore the early detection of ALDH activity levels can be prognostic and guide the therapeutic strategies. MyBioSource's PicoProbe Aldehyde Dehydrogenase Activity Assay Kit is a robust tool to quantify ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form the NADH which couples with the PicoProbe to generate a potent fluorescence (Ex/Em = 535/587). The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Application- The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
Background: The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis. Therefore the early detection of ALDH activity levels can be prognostic and guide the therapeutic strategies. MyBioSource's PicoProbe Aldehyde Dehydrogenase Activity Assay Kit is a robust tool to quantify ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form the NADH which couples with the PicoProbe to generate a potent fluorescence (Ex/Em = 535/587). The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Application- The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
Product Categories/Family for PicoProbe Aldehyde Dehydrogenase assay kit
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Product Notes
The PicoProbe Aldehyde Dehydrogenase (Catalog #AAA841670) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PicoProbe Aldehyde Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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