Rabbit anti-Human, Mouse UCMA Polyclonal Antibody | anti-UCMA antibody

UCMA Antibody

Cat. Num. AAA150184

• Gene Names: UCMA; GRP; C10orf49; GRP/UCMA
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: UCMA Antibody is affinity chromatography purified via peptide column.
• Synonyms: UCMA; Polyclonal Antibody; UCMA Antibody; UCMA Antibody: GRP; C10orf49; Unique cartilage matrix-associated protein; Gla-rich protein; Ucma-C; anti-UCMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: UCMA Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; UCMA Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-UCMA antibody: ELISA, Western Blot (WB)
• Application Notes: UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 ug/mL.; ; Antibody validated: Western Blot in human samples. ; ; All other applications and species not yet tested.; Optimal dilutions for each application to be determined by the researcher.
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (81%)
• Immunogen: UCMA antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human UCMA. ; The immunogen is located within the last 50 amino acids of UCMA.
• Positive Control: 1) Cat. No. MBS151599 - SW1353 Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: UCMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. ; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of UCMA in SW1353 cell lysate with UCMA antibody at 2.5 μg/mL.)

Related Product Information for anti-UCMA antibody

UCMA Antibody: UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 50400088
• NCBI GeneID: 221044
• NCBI Accession #: NP_660357
• NCBI GenBank Nucleotide #: NM_145314.2
• UniProt Accession #: Q8WVF2
• Molecular Weight: Predicted: 15 kDa; Observed: 19 kDa
• NCBI Official Full Name: unique cartilage matrix-associated protein isoform 1 preproprotein
• NCBI Official Synonym Full Names: upper zone of growth plate and cartilage matrix associated
• NCBI Official Symbol: UCMA
• NCBI Official Synonym Symbols: GRP; C10orf49; GRP/UCMA
• NCBI Protein Information: unique cartilage matrix-associated protein; Gla-rich protein
• UniProt Protein Name: Unique cartilage matrix-associated protein
• Protein Family: Unique cartilage matrix-associated protein
• UniProt Gene Name: UCMA
• UniProt Synonym Gene Names: C10orf49; Ucma-C; GRP
• UniProt Entry Name: UCMA_HUMAN

NCBI Description

This gene encodes a chondrocyte-specific, highly charged protein that is abundantly expressed in the upper immature zone of fetal and juvenile epiphyseal cartilage. The encoded protein undergoes proteolytic processing to generate a mature protein that is secreted into the extracellular matrix. The glutamic acid residues in the encoded protein undergo gamma carboxylation in a vitamin K-dependent manner. Undercarboxylation of the encoded protein is associated with osteoarthritis in humans. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Uniprot Description

UCMA: May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface. Belongs to the UCMA family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: proteinaceous extracellular matrix; extracellular space; perinuclear region of cytoplasm

Biological Process: negative regulation of osteoblast differentiation