Adenosine Deaminase Assay Kit | ADA assay kit

Adenosine Deaminase Activity Assay Kit (Fluorometric)

Cat. Num. AAA841490

• Synonyms: Adenosine Deaminase; Adenosine Deaminase Activity Assay Kit (Fluorometric); ADA Activity Assay Kit (Fluorometric); ADA assay kit

• Sequence Length: 363

• Application Notes: Detection of Adenosine Deaminase activity
• Samples: Cell and tissue lysate; Nuclear Extract; Cell Medium; Purified recombinant protein
• Detection Method: Fluorescence (Ex/Em = 535/587 nm)
• Features & Benefits: Rapid, and convenient ; The kit measures total Activity of Adenosine Deaminase with limit of quantification 10 uU recombinant Adenosine Deaminase. Range of the assay: 10-100 uU.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADA assay kit

Product Information: Adenosine Deaminase Activity Assay Kit (Fluorometric): Simple & convenient assay to measure ada activity in a variety of samples. Limit of quantification: 10 uU recombinant Adenosine Deaminase. Assay range: 10-100 uU. 100 assay

Background: Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In MyBioSource's ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 uU recombinant Adenosine Deaminase. The range of the assay is from 10-100 uU.

Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application- Detection of Adenosine Deaminase activity

Product Categories/Family for ADA assay kit

Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z); Epigenetics; Other Epigenetics Products; Assay Kits

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.2
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: Adenosine deaminase
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: EC 3.5.4.4; Nucleotide Metabolism - purine; Hydrolase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cell junction; cytosol; external side of plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; adenosine catabolic process; T cell activation; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; positive regulation of T cell differentiation in the thymus; histamine secretion; purine ribonucleoside monophosphate biosynthetic process; response to vitamin E; regulation of cell-cell adhesion mediated by integrin; negative regulation of mature B cell apoptosis; positive regulation of T cell receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of germinal center formation; hypoxanthine salvage; negative regulation of adenosine receptor signaling pathway; positive regulation of smooth muscle contraction; embryonic gut development; aging; placenta development; Peyer's patch development; response to drug; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; response to hydrogen peroxide; xanthine biosynthetic process; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency